Mackiewicz-Milewska Magdalena, Grześk Elżbieta, Kroszczyński Andrzej C, Cisowska-Adamiak Małgorzata, Mackiewicz-Nartowicz Hanna, Baran Lilianna, Szymkuć-Bukowska Iwona, Wiciński Michał, Hagner Wojciech, Grześk Grzegorz
Department of Rehabilitation Collegium Medicum in Bydgoszcz Faculty of Health Science, Nicolaus Copernicus University in Toruń, Poland.
Department of Pediatrics, Hematology and Oncology Collegium Medicum in Bydgoszcz Faculty of Medicine, Nicolaus Copernicus University in Toruń, Poland.
Adv Med Sci. 2018 Mar;63(1):64-67. doi: 10.1016/j.advms.2017.06.002. Epub 2017 Aug 16.
The mechanism of action of low level laser irradiation on tissues is unclear. Authors of publications present the positive clinical impact of low and medium power laser irradiation on vascular reactivity. The purpose of this study was to analyze the role of vascular endothelium in laser-induced constricted by endothelin-1 and phenylephrine.
Experiments were performed on isolated and perfused rat tail arteries of weighing 250-350g male Wistar rats. Contractility of arteries as a response to endothelin-1 and phenylephrine was measured after exposure to laser stimulation (10, 30 and 110mW).
Laser irradiation inhibits vascular smooth muscle contraction induced by endothelin-1 and an alpha-adrenergic receptor agonist, phenylephrine proportionally to the laser power. Concentration-response curves were shifted to the right with significant reduction in maximal response. Laser irradiation at the power of 10mW, 30mW, and 110mW reduced the maximum response of arteries stimulated with phenylephrine sequentially to 88%, 72%, and 52%. Similar findings were observed during stimulation of endothelin-1. Laser irradiation at the power of 10mW, 30mW and 110mW resulted in maximal response respectively reduced to 94%, 62% and 38%.
Our results strongly suggest that during low level laser irradiation vascular smooth muscle cells reactivity is reduced, this effect is present in arteries with normal endothelium. The mechanism of action of laser biosimulation on tissues is unclear. Authors of publications present the positive clinical impact of low level laser irradiation on vascular reactivity.
低强度激光照射对组织的作用机制尚不清楚。出版物的作者提出了低功率和中功率激光照射对血管反应性的积极临床影响。本研究的目的是分析血管内皮在激光诱导的由内皮素 -1 和去氧肾上腺素引起的血管收缩中的作用。
实验在体重 250 - 350g 的雄性 Wistar 大鼠的离体灌注尾动脉上进行。在暴露于激光刺激（10、30 和 110mW）后，测量动脉对内皮素 -1 和去氧肾上腺素的收缩反应。
激光照射抑制由内皮素 -1 和α - 肾上腺素能受体激动剂去氧肾上腺素诱导的血管平滑肌收缩，其抑制程度与激光功率成比例。浓度 - 反应曲线向右移动，最大反应显著降低。10mW、30mW 和 110mW 功率的激光照射分别使去氧肾上腺素刺激的动脉最大反应依次降低至 88%、72%和 52%。在内皮素 -1 刺激期间观察到类似结果。10mW、30mW 和 110mW 功率的激光照射分别使最大反应降低至 94%、62%和 38%。
我们的结果强烈表明，在低强度激光照射期间，血管平滑肌细胞反应性降低，这种效应存在于内皮正常的动脉中。激光生物模拟对组织的作用机制尚不清楚。出版物的作者提出了低强度激光照射对血管反应性的积极临床影响。
