Department of Radiation Oncology, Zhongshan Hospital Affiliated Xiamen University, Xiamen, China.
Eur Rev Med Pharmacol Sci. 2019 Dec;23(23):10324-10331. doi: 10.26355/eurrev_201912_19670.
Oral squamous cell carcinoma (OSCC), the most frequent head and neck cancer, has a high potential for metastasis. MiR-126 plays an important role in the tumorigenesis of many tumors; however, there were little studies in OSCC. The purpose of our study was to explore how miR-126 and ADAM9 worked on migration and invasion in OSCC.
The quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was applied to detect the mRNA level of miR-126 and ADAM9. The transwell assay was utilized to calculate the migratory and invasive capacities in the OSCC cells. The luciferase report assay was utilized to verify that ADAM9 was a direct target of miR-126.
MicroR-126 was downregulated in OSCC tissues and cell lines SCC25 and HSC3. ADAM9 was predicted to be a direct target of miR-126 and was upregulated in the OSCC cells. In addition, miR-126 suppressed the migratory and invasive ability via mediating the expression of ADAM9 by directly targeting its mRNA 3'-noncoding region (UTR), whose partial functions was reversed by ADAM9.
MiR-126 inhibited the migratory and invasive capacities of OSCC by directly targeting the 3'-UTR of ADAM9 mRNA. It is suggested that miR-126/ADAM9 axis may play an essential role in inhibiting the abilities of migration and invasion in oral squamous cell carcinoma cells.
口腔鳞状细胞癌（OSCC）是最常见的头颈部癌症，具有很高的转移潜力。miR-126 在许多肿瘤的发生发展中起着重要作用，但在 OSCC 中研究甚少。本研究旨在探讨 miR-126 和 ADAM9 如何影响 OSCC 的迁移和侵袭。
采用实时定量聚合酶链反应（qRT-PCR）检测 miR-126 和 ADAM9 的 mRNA 水平。Transwell 实验检测 OSCC 细胞的迁移和侵袭能力。荧光素酶报告实验验证 ADAM9 是 miR-126 的直接靶基因。
miR-126 在 OSCC 组织和 SCC25、HSC3 细胞系中下调。ADAM9 被预测为 miR-126 的直接靶基因，在 OSCC 细胞中上调。此外，miR-126 通过直接靶向其 mRNA 3'-非编码区（UTR）来抑制 ADAM9 的表达，从而抑制 OSCC 细胞的迁移和侵袭能力，其部分功能可被 ADAM9 逆转。
miR-126 通过直接靶向 ADAM9 mRNA 的 3'-UTR 抑制 OSCC 的迁移和侵袭能力。提示 miR-126/ADAM9 轴可能在抑制口腔鳞状细胞癌细胞迁移和侵袭能力中发挥重要作用。
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