非洲大蜗牛（Archachatina marginata）血淋巴中多种形式酸性磷酸酶的展示。

Demonstration of multiple forms of acid phosphatase in hemolymph of the African snail, Archachatina marginata.

Ebong B, Glew R H

Department of Biochemistry, University of Benin, Nigeria, West Africa.

Comp Biochem Physiol B. 1979;63(3):315-22. doi: 10.1016/0305-0491(79)90255-4.

Abstract

Hemolymph from the giant African snail Archachatina marginata has been analyzed for its content of certain lysosomal hydrolases and shown to contain substantial quantities of acid phosphatase (285 units/ml) hexosaminidase (512 units per ml) and beta-glucuronidase (28 units/ml). 2. Hemolymph acid phosphatase can be fractionated into 6 active components by DEAE-Sephadex chromatography. 3. Some of the acid phosphatase species can be distinguished on the basis of heat stability, pH dependency and sensitivity to inhibitors including phosphate, L(+) tartrate, fluoride, formaldehyde and 1.10 phenanthroline.

摘要

对非洲大蜗牛（Archachatina marginata）的血淋巴进行了分析，检测了其中某些溶酶体水解酶的含量，结果显示其含有大量酸性磷酸酶（285单位/毫升）、己糖胺酶（512单位/毫升）和β-葡萄糖醛酸酶（28单位/毫升）。2. 通过DEAE-葡聚糖凝胶色谱法可将血淋巴酸性磷酸酶分离为6种活性成分。3. 部分酸性磷酸酶种类可根据热稳定性、pH依赖性以及对包括磷酸盐、L(+)酒石酸盐、氟化物、甲醛和1,10-菲啰啉在内的抑制剂的敏感性来区分。