Nanotechnology. 2020 Apr 3;31(14):145301. doi: 10.1088/1361-6528/ab6236. Epub 2019 Dec 16.
To overcome the shortcoming of drug-loaded nanoparticles, such as high initial burst release and wide size distribution, a novel manufacturing technique for core-shell structure nanoparticle was developed by combining microfluidic chip and electrohydrodynamic atomization. In this study, the mixture solution of the surfactant 1, 2- dipalmitoyl-sn-glycero-3-phosphoglycerol and the polymeric coating material polylactic-glycolic-acid was introduced into the outer microchannel of the microfluidic chip as the particle's shell. And the encapsulated drug paclitaxel was pumped into the inner microchannel as the core. Then, the particles with a nanoscale-size core-shell structure were generated by applying an electric field on the laminar flow which was formed in the microfluidic chip. Operation parameters, including working voltage, carrier material and surfactant concentration as well as liquid flow rates were optimized for nanoparticles generation. The properties of drug-loaded nanoparticles in terms of their particle size, zeta potential and encapsulation efficiency were investigated. Under the optimal experimental conditions, the particle size was approximately 145 nm and encapsulation efficiency reached 92%. Moreover, the drug release of these nanoparticles could be prolonged over a significant period for more than ten days. It can be expected that this innovative approach could provide a useful platform for drug-loaded core-shell nanoparticles developing.
为了克服载药纳米粒子的缺点，如初始突释和较宽的粒径分布，本研究结合微流控芯片和电动力学雾化技术，开发了一种新型核壳结构纳米粒子的制造技术。在本研究中，将表面活性剂 1,2-二棕榈酰-sn-甘油-3-磷酸甘油和聚合包衣材料聚乳酸-羟基乙酸混合溶液引入微流控芯片的外通道作为粒子的壳。将包裹的药物紫杉醇作为核泵入微流控芯片的内通道。然后，在外通道和内通道之间的层流上施加电场，生成具有纳米级核壳结构的粒子。优化了操作参数，包括工作电压、载体材料和表面活性剂浓度以及液体流速，以实现纳米粒子的生成。研究了载药纳米粒子的粒径、Zeta 电位和包封效率等性质。在最佳实验条件下，粒子的粒径约为 145nm，包封效率达到 92%。此外，这些纳米粒子的药物释放可以延长十几天以上。可以预期，这种创新方法可为载药核壳纳米粒子的开发提供一个有用的平台。
