Activity of some dehydrogenase enzymes in mitochondria from Physarum polycephalum.
作者信息
McCarthy J L, Nations C
机构信息
Department of Biology, Southern Methodist University, Dallas, Texas 75275.
出版信息
Comp Biochem Physiol B. 1979;63(4):495-9. doi: 10.1016/0305-0491(79)90052-x.
PMID:318427
Abstract
- Coupled mitochondria were isolated from exponentially growing Physarum polycephalum. 2. Activity of malate dehydrogenase (oxalacetate reduction) was 10.9 mumol/min/mg protein; the apparent Km was 64 microM. 3. The activity of NADP-isocitric dehydrogenase (IDH) was 110 nmol/min/mg with apparent Km of 35 microM. 4. NAD-IDH showed allosteric properties with AMP as a positive modulator. The apparent Km for the unmodulated activity, 2 mM, was decreased to 0.95 mM by 0.13 mM AMP. 5. Succinic dehydrogenase activity was estimated as three times higher than that of alpha-glycerophosphate dehydrogenase. 6. Mitochondria contained significant amounts of phenolic compounds. Protein estimation by the Bradford method is recommended.
摘要
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