Cell and Matrix Research Institute, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea.
J Ethnopharmacol. 2020 Mar 25;250:112484. doi: 10.1016/j.jep.2019.112484. Epub 2019 Dec 13.
A promising approach to treat a variety of diseases are considered as complementary and alternative herbal medicines. Prunus serrulata var. spontanea L. (Rosaceae) is used as herbal medicine to treat allergic diseases according to the Donguibogam, a tradition medical book of the Joseon Dynasty in Korea.
We prepared the aqueous extract of the bark of P. serrulata (AEBPS) and aimed to investigate the effects in mouse anaphylaxis models and various types of mast cells, including RBL-2H3, primary cultured peritoneal and bone marrow-derived mast cells.
We used ovalbumin (OVA)-induced active systemic anaphylaxis (ASA) and immunoglobulin (Ig) E-mediated passive cutaneous anaphylaxis (PCA) models, in vivo. The control drug dexamethasone (10 mg/kg) was used to compare the effectiveness of AEBPS (1-100 mg/kg). In vitro, IgE-stimulated mast cells were used to confirm the role of AEBPS (1-100 μg/mL). For statistical analyses, p values less than 0.05 were considered to be significant.
In ASA model, oral administration of AEBPS suppressed the hypothermia and increased level of serum histamine in a dose-dependent manner. AEBPS attenuated the serum IgE, OVA-specific IgE, and interleukin (IL)-4. Oral administration of AEBPS also blocked mast cell-dependent PCA. AEBPS suppressed degranulation of mast cells by reducing intracellular calcium level in mast cells. AEBPS inhibited tumor necrosis factor-α and IL-4 expression and secretion in a concentration-dependent manner through the reduction of nuclear factor-κB.
On the basis of these findings, AEBPS could serve as a potential therapeutic target for the management of mast cell-mediated allergic inflammation and as a regulator of mast cell activation.
根据韩国朝鲜王朝传统医学书籍《东医宝鉴》，野巴旦杏（蔷薇科）的树皮用作草药治疗过敏性疾病，被认为是一种有前途的治疗各种疾病的方法，包括补充和替代草药医学。
我们制备了野巴旦杏树皮的水提取物（AEBPS），并旨在研究其在小鼠过敏反应模型和各种类型肥大细胞中的作用，包括 RBL-2H3、原代培养的腹腔和骨髓来源的肥大细胞。
我们使用卵清蛋白（OVA）诱导的主动全身性过敏反应（ASA）和免疫球蛋白（Ig）E 介导的被动皮肤过敏反应（PCA）模型，在体内进行研究。将对照药物地塞米松（10mg/kg）用于比较 AEBPS（1-100mg/kg）的有效性。在体外，使用 IgE 刺激的肥大细胞来确认 AEBPS（1-100μg/mL）的作用。对于统计分析，p 值小于 0.05 被认为具有统计学意义。
在 ASA 模型中，AEBPS 的口服给药以剂量依赖性方式抑制体温过低和血清组胺水平升高。AEBPS 减弱了血清 IgE、OVA 特异性 IgE 和白细胞介素（IL）-4。AEBPS 还通过抑制肥大细胞依赖性 PCA 来阻断肥大细胞。AEBPS 通过降低肥大细胞内的钙水平来抑制脱颗粒。AEBPS 通过减少核因子-κB 抑制肿瘤坏死因子-α和 IL-4 的表达和分泌。
基于这些发现，AEBPS 可以作为治疗肥大细胞介导的过敏炎症的潜在治疗靶点，并作为肥大细胞活化的调节剂。
