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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Department of Zoophysiology, University of Göteborg, Sweden.

Comp Biochem Physiol B. 1979;62(3):275-9. doi: 10.1016/0305-0491(79)90213-x.

Microtubules (MT) from a guinea pig brain 25,000 g supernatant are not depolymerized by colchicine in contrast to MT from similar preparations of rat and rabbit. 2. The colchicine-stability was lost if the guinea pig brain homogenate was centrifuged at a higher g-level, further purified or if only the grey matter was used. 3. The association constant of colchicine to tubulin did not differ between a stable and a labile guinea pig brain preparation. 4. The GTP-hydrolysis was higher in the guinea pig preparation containing stable MT, than in the preparation containing labile MT. Additional GTP added to the polymerized MT before colchicine exposure, labilized the MT. Preincubation with NaF decreased the GTP-hydrolysis and caused a colchicine depolymerization. 5. The results indicate species differences in colchicine sensitivity of in vitro polymerized MT, probably depending on differences in GTP-hydrolysis.

与大鼠和兔子类似制剂中的微管不同，豚鼠脑25,000 g上清液中的微管（MT）不会被秋水仙碱解聚。2. 如果豚鼠脑匀浆以更高的g值离心、进一步纯化或仅使用灰质，则秋水仙碱稳定性会丧失。3. 稳定和不稳定的豚鼠脑制剂中秋水仙碱与微管蛋白的结合常数没有差异。4. 含有稳定微管的豚鼠制剂中的GTP水解高于含有不稳定微管的制剂。在秋水仙碱暴露前向聚合微管中添加额外的GTP会使微管不稳定。用NaF预孵育会降低GTP水解并导致秋水仙碱解聚。5. 结果表明体外聚合微管对秋水仙碱的敏感性存在种属差异，可能取决于GTP水解的差异。

Department of Zoophysiology, University of Göteborg, Sweden.

Comp Biochem Physiol B. 1979;62(3):275-9. doi: 10.1016/0305-0491(79)90213-x.

Microtubules (MT) from a guinea pig brain 25,000 g supernatant are not depolymerized by colchicine in contrast to MT from similar preparations of rat and rabbit. 2. The colchicine-stability was lost if the guinea pig brain homogenate was centrifuged at a higher g-level, further purified or if only the grey matter was used. 3. The association constant of colchicine to tubulin did not differ between a stable and a labile guinea pig brain preparation. 4. The GTP-hydrolysis was higher in the guinea pig preparation containing stable MT, than in the preparation containing labile MT. Additional GTP added to the polymerized MT before colchicine exposure, labilized the MT. Preincubation with NaF decreased the GTP-hydrolysis and caused a colchicine depolymerization. 5. The results indicate species differences in colchicine sensitivity of in vitro polymerized MT, probably depending on differences in GTP-hydrolysis.

与大鼠和兔子类似制剂中的微管不同，豚鼠脑25,000 g上清液中的微管（MT）不会被秋水仙碱解聚。2. 如果豚鼠脑匀浆以更高的g值离心、进一步纯化或仅使用灰质，则秋水仙碱稳定性会丧失。3. 稳定和不稳定的豚鼠脑制剂中秋水仙碱与微管蛋白的结合常数没有差异。4. 含有稳定微管的豚鼠制剂中的GTP水解高于含有不稳定微管的制剂。在秋水仙碱暴露前向聚合微管中添加额外的GTP会使微管不稳定。用NaF预孵育会降低GTP水解并导致秋水仙碱解聚。5. 结果表明体外聚合微管对秋水仙碱的敏感性存在种属差异，可能取决于GTP水解的差异。