College of Chemical Engineering, Fuzhou University, Fuzhou, Fujian, 350108, China; College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China.
College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China; Yango University, Fuzhou, Fujian, 350015, China.
Food Chem Toxicol. 2020 Feb;136:111056. doi: 10.1016/j.fct.2019.111056. Epub 2019 Dec 14.
Cold stage adversely affects cell proliferation and cell viability of probiotics such as Streptococcus thermophilus in food industry, new type of cryoprotectants continues to be needed. Gelatin-based antifreeze peptide becomes a popular topic because of its cryoprotective effects on cold-stressed probiotics. In this study the effects of tilapia scales antifreeze peptides (TSAPP) on cell viability and oxidant stress of S. thermophilus during cold stage were investigated. The results showed that the percentage of viable cells was increased 10.85 folds compared with control groups. Addition of TSAPP activated the activities of ATPases, relieved the hyperpolarization of cell membrane potential and regulated the intracellular Ca concentration. Furthermore, TSAPP significantly inhibited reactive oxygen species level and malonaldehyde content in cells. Under cryopreservation with TSAPP, cells of S. thermophilus maintained higher activities of antioxidant enzymes including catalase, peroxidase and total antioxidant capacity. These findings indicate that TSAPP likely offered its cellular protection by maintaining membrane integrity and alleviation of oxidative stress.
在食品工业中，冷阶段会对益生菌（如嗜热链球菌）的细胞增殖和细胞活力产生不利影响，因此需要继续开发新型的冷冻保护剂。基于明胶的抗冻肽因其对冷应激益生菌的保护作用而成为研究热点。本研究探讨了罗非鱼鳞抗冻肽（TSAPP）对冷阶段嗜热链球菌细胞活力和氧化应激的影响。结果表明，与对照组相比，活细胞的百分比增加了 10.85 倍。添加 TSAPP 可激活 ATP 酶的活性，缓解细胞膜电位的超极化，并调节细胞内 Ca 浓度。此外，TSAPP 可显著抑制细胞内活性氧水平和丙二醛含量。在使用 TSAPP 进行冷冻保存时，嗜热链球菌的细胞保持更高的抗氧化酶活性，包括过氧化氢酶、过氧化物酶和总抗氧化能力。这些发现表明，TSAPP 可能通过维持膜完整性和减轻氧化应激来提供细胞保护。
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