Osorio Clarice Fraga Esteves Maciel, Costa Waldemar Silva, Gallo Carla Braga Mano, Sampaio Francisco José Barcellos
Fellow PhD degree, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, Universidade do Estado do Rio de Janeiro (UERJ), Brazil. Conception and design of the study; acquisition, analysis and interpretation of data; technical procedures; histological examinations; statistics analysis; manuscript preparation and writing; final approval.
PhD, Associate Professor, Urogenital Research Unit, UERJ, Rio de Janeiro-RJ, Brazil. Conception and design of the study, technical procedures, histological examination, interpretation of data, manuscript preparation and writing, final approval.
Acta Cir Bras. 2019 Dec 13;34(10):e201901005. doi: 10.1590/s0102-865020190100000005. eCollection 2019.
To quantify and compare the expression of stromal elements in prostate adenocarcinoma of different Gleason scores with non-tumor area (control).
We obtained 132 specimens from samples of prostate peripheral and transition zone. We analyzed the following elements of the extracellular matrix: collagen fibers, elastic system, smooth muscle fibers and blood vessels. The tumor area and non-tumor area (control) of the TMA (tissue microarray) were photographed and analyzed using the ImageJ software.
The comparison between the tumor area and the non-tumor area showed significant differences between stromal prostate elements. There was an increase of collagen fibers in the tumor area, mainly in Gleason 7. Elastic system fibers showed similar result, also from the Gleason 7. Blood vessels showed a significant increase occurred in all analyzed groups. The muscle fibers exhibited a different behavior, with a decrease in relation to the tumor area.
There is a significant difference between the extracellular matrix in prostate cancer compared to the non-tumor area (control) especially in Gleason 7. Important modifications of the prostatic stromal elements strongly correlate with different Gleason scores and can contribute to predict the pathological staging of prostate cancer.
量化并比较不同Gleason评分的前列腺腺癌组织与非肿瘤区域（对照）中基质成分的表达。
我们从前列腺外周区和移行区样本中获取了132个标本。我们分析了细胞外基质的以下成分：胶原纤维、弹性系统、平滑肌纤维和血管。使用ImageJ软件对组织微阵列（TMA）的肿瘤区域和非肿瘤区域（对照）进行拍照和分析。
肿瘤区域与非肿瘤区域的比较显示，前列腺基质成分存在显著差异。肿瘤区域的胶原纤维增加，主要在Gleason 7级中。弹性系统纤维也呈现类似结果，同样始于Gleason 7级。血管在所有分析组中均显著增加。肌纤维表现出不同的变化趋势，相对于肿瘤区域有所减少。
前列腺癌的细胞外基质与非肿瘤区域（对照）之间存在显著差异，尤其是在Gleason 7级中。前列腺基质成分的重要改变与不同的Gleason评分密切相关，并有助于预测前列腺癌的病理分期。
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