Alves Edilaine F, de Freitas Ribeiro Bruno Leonardo M, Costa Waldemar S, Gallo Carla B M, Sampaio Francisco J B
Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Prostate. 2018 Mar;78(4):289-293. doi: 10.1002/pros.23472. Epub 2018 Jan 8.
McNeal divided the human prostate into three major anatomical areas: the peripheral zone (PZ), the central zone (CZ), and the transition zone (TZ). Each of these areas is biologically and histologically distinct. The PZ and TZ have clinical significance and are associated with prostate cancer (PC) and benign prostatic hyperplasia (BPH), respectively. Therefore, the objective of the present study was to quantitatively and qualitatively analyze the parenchymal and stromal components that constitute the different prostate zones.
We assessed 19 samples from each prostate zone. The samples were obtained from necropsies of young people between 18 and 32 years of age with intact urogenital tracts. The samples were fixed in 4% buffered formalin and processed for paraffin embedding. Sections with a thickness of five micrometres were obtained from each sample. The sections were stained using histochemical and immunohistochemical techniques to identify the acinar and stromal components of each zone. Photomicrographs were obtained for morphometric analysis using an algorithm based on color segmentation. Data were analyzed using one-way analysis of variance (ANOVA) with the Bonferroni post-test. Differences with P < 0.05 were regarded as statistically significant.
Collagen fibres were more numerous in the TZ (+40.26%; P = 0.0230) than in the PZ. Muscle fibres were also more numerous in the TZ (+47.05%; P = 0.0120) than in the PZ. Elastic system fibres in the TZ significantly differed from those in the PZ (+84.61%; P = 0.0012) and the CZ (+61.66%; P = 0.0074). Similarly, nerves in the PZ (-42.86%; P = 0.0107) significantly differed from nerves in the CZ. Epithelial height was lower in the TZ than in the PZ (-30.17%; P = 0.0034) and the CZ (-25.01%; P = 0.0330).
Our objective, quantitative data regarding the various elements that constitute the normal prostate stroma allowed us to reveal differences among prostate zones. This study established patterns for normal parameters and may be used for posterior comparisons in histopathological analysis.
麦克尼尔将人类前列腺分为三个主要解剖区域：外周区（PZ）、中央区（CZ）和移行区（TZ）。这些区域在生物学和组织学上各有不同。外周区和移行区具有临床意义，分别与前列腺癌（PC）和良性前列腺增生（BPH）相关。因此，本研究的目的是对构成不同前列腺区域的实质和间质成分进行定量和定性分析。
我们评估了每个前列腺区域的19个样本。样本取自18至32岁泌尿生殖道完整的年轻人的尸检。样本用4%缓冲福尔马林固定并进行石蜡包埋处理。从每个样本中获取厚度为5微米的切片。使用组织化学和免疫组织化学技术对切片进行染色，以识别每个区域的腺泡和间质成分。使用基于颜色分割的算法获取显微照片进行形态计量分析。数据采用单因素方差分析（ANOVA）和Bonferroni事后检验进行分析。P<0.05的差异被视为具有统计学意义。
移行区的胶原纤维比外周区多（+40.26%；P=0.0230）。移行区的肌纤维也比外周区多（+47.05%；P=0.0120）。移行区的弹性系统纤维与外周区（+84.61%；P=0.0012）和中央区（+61.66%；P=0.0074）有显著差异。同样，外周区的神经与中央区的神经有显著差异（-42.86%；P=0.0107）。移行区的上皮高度低于外周区（-30.17%；P=0.0034）和中央区（-25.01%；P=0.0330）。
我们关于构成正常前列腺间质的各种成分的客观定量数据使我们能够揭示前列腺区域之间的差异。本研究建立了正常参数模式，可用于组织病理学分析中的后续比较。
