Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh.
Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, Bangladesh.
J Mol Microbiol Biotechnol. 2019;29(1-6):43-56. doi: 10.1159/000504511. Epub 2019 Dec 18.
The human gut is inhabited by several hundred different bacterial species. These bacteria are closely associated with our health and well-being. The composition of these diverse commensals is influenced by our dietary intakes. Non-caloric artificial sweeteners (NAS) have gained global popularity, particularly among diabetic patients, due to their perceived health benefits, such as reduction of body weight and maintenance of blood glucose level compared to caloric sugars. Recent studies have reported that these artificial sweeteners can alter the composition of gut microbiota and, thus, affect our normal physiological state. Here, we investigated the effect of aspartame and acesulfame potassium (ace-K), two popular NAS, in a commercial formulation on the growth and metabolic pathways of omnipresent gut commensal Escherichia coliby analyzing the relative expression levels of the key genes, which control over twenty important metabolic pathways. Treatment with NAS preparation (aspartame and ace-K) modulates the growth of E. colias well as inducing the expression of important metabolic genes associated with glucose (pfkA, sucA, aceE, pfkB, lpdA), nucleotide (tmk, adk, tdk, thyA), and fatty acid (fabI) metabolisms, among others. Several of the affected geneswere previously reported to be important for the colonization of the microbes in the gut. These findings may shed light on the mechanism of alteration of gut microbes and their metabolism by NAS.
人类肠道中栖息着数百种不同的细菌物种。这些细菌与我们的健康和幸福密切相关。这些多样化的共生菌的组成受到我们饮食摄入的影响。非热量人工甜味剂（NAS）由于其被认为的健康益处，如与热量糖相比减轻体重和维持血糖水平，在全球范围内受到欢迎，尤其是在糖尿病患者中。最近的研究报告称，这些人工甜味剂可以改变肠道微生物群的组成，从而影响我们的正常生理状态。在这里，我们通过分析控制二十多种重要代谢途径的关键基因的相对表达水平，研究了一种商业配方中两种流行的 NAS（阿斯巴甜和乙酰磺胺酸钾）对普遍存在的肠道共生菌大肠杆菌生长和代谢途径的影响。NAS 制剂（阿斯巴甜和乙酰磺胺酸钾）的处理不仅调节大肠杆菌的生长，还诱导与葡萄糖（pfkA、sucA、aceE、pfkB、lpdA）、核苷酸（tmk、adk、tdk、thyA）和脂肪酸（fabI）代谢等相关的重要代谢基因的表达。其中一些受影响的基因先前被报道对微生物在肠道中的定植很重要。这些发现可能揭示了 NAS 改变肠道微生物及其代谢的机制。
