锌转运蛋白在参与锌稳态的功能不同组织中的差异表达。
Differential expression of zinc transporters in functionally contrasting tissues involved in zinc homeostasis.
作者信息
Alluri Kiran, Nair Krishna Pillay Madhavan, Ghosh Sudip
机构信息
Molecular Biology Division, ICMR-National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, India.
Micronutrient Research Group, ICMR- National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, India.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2020;39(4):615-629. doi: 10.1080/15257770.2019.1670838. Epub 2019 Dec 18.
Zinc homeostasis is maintained by 24 tissue-specific zinc transporters which include ZnTs (ZnT1-10), ZIPs (ZIP1-14), in addition to metallothionein (MT). Current study aimed the role of zinc transporters in maintaining the basal levels of zinc in functionally contrasting tissue specific THP-1 (monocyte), RD (muscle), and Saos-2 (bone) cells. Zinc transporters expression was assessed by qRT-PCR. The mRNA levels of ZnTs (ZnT5-7 & ZnT9), ZIPs (ZIP6-10, ZIP13-14), and MT were significantly ( < 0.05) higher in Saos-2 compared to THP-1 and RD. The present study suggests that distinct expression pattern of zinc transporters and metallothionein might be responsible for the differential zinc assimilation.
锌稳态由24种组织特异性锌转运蛋白维持，其中包括锌转运体（ZnT1 - 10）、锌铁调控蛋白（ZIP1 - 14），此外还有金属硫蛋白（MT）。当前研究旨在探讨锌转运蛋白在功能不同的组织特异性THP - 1（单核细胞）、RD（肌肉）和Saos - 2（骨）细胞中维持锌基础水平的作用。通过定量逆转录聚合酶链反应（qRT - PCR）评估锌转运蛋白的表达。与THP - 1和RD相比，Saos - 2中锌转运体（ZnT5 - 7和ZnT9）、锌铁调控蛋白（ZIP6 - 10、ZIP13 - 14）和金属硫蛋白的mRNA水平显著更高（<0.05）。本研究表明，锌转运蛋白和金属硫蛋白的不同表达模式可能是锌同化差异的原因。
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