Nagasaka A, Yoshida S, Nakai A, Ohyama T, Iwase K, Ohtani S, Shinoda S, Masunaga R, Nakagawa H
Department of Internal Medicine and Surgery, Fujita-Gakuen Health University School of Medicine, Aichi, Japan.
Metabolism. 1988 Nov;37(11):1051-4. doi: 10.1016/0026-0495(88)90066-2.
We have shown that the level of DNA polymerase beta of rat adrenal cortex is regulated by pituitary trophic hormones and may correlate with their endocrine function. Here we measured DNA polymerase beta activity in human thyroid tissues of various benign and malignant thyroid disorders in order to verify the correlation between DNA polymerase beta activity and endocrine function. In Graves' disease (hyperfunction), the level of DNA polymerase beta per cell was three times higher than in normal thyroid, while in undifferentiated thyroid carcinomas this enzyme level was lower than normal. Furthermore, DNA polymerase beta in the crude extracts of cancer cells showed larger molecular forms, ranging from five to 12S, upon sucrose gradient sedimentation. These observations further support the hypothesis that the activity of DNA polymerase beta correlates, in part, with the functional level of the endocrine organ and with cell differentiation.
我们已经表明，大鼠肾上腺皮质中DNA聚合酶β的水平受垂体促激素调节，并且可能与其内分泌功能相关。在此，我们测定了各种良性和恶性甲状腺疾病患者甲状腺组织中的DNA聚合酶β活性，以验证DNA聚合酶β活性与内分泌功能之间的相关性。在格雷夫斯病（功能亢进）中，每个细胞的DNA聚合酶β水平比正常甲状腺高两倍，而在未分化甲状腺癌中，这种酶的水平低于正常。此外，癌细胞粗提物中的DNA聚合酶β在蔗糖梯度沉降时显示出更大的分子形式，范围从5到12S。这些观察结果进一步支持了这样的假设，即DNA聚合酶β的活性部分与内分泌器官的功能水平和细胞分化相关。
