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Amphibian intestinal brush border membranes-I. Isolation from Rana catesbeiana tadpole.

作者信息

Dauca M, Hourdry J, Hugon J S, Menard D

机构信息

Laboratoire de Biologie et Biochimie du Développement, Ecole Normale Supérieure, Saint-Cloud, France.

出版信息

Comp Biochem Physiol B. 1979;64(2):155-9. doi: 10.1016/0305-0491(79)90154-8.

Abstract
  1. Intestinal brush border membrane vesicles have been isolated form Rana catesbeiana tadpole. 2. Electron microscopy of brush border membrane vesicles demonstrates a fairly homogenous preparation of vesicles, some of them still containing electron dense material. 3. The dense vesicles probably comprise both microvillus core and membrane. 4. Negative staining of vesicles reveals the presence of knob-like structures (particles) covering the outer surface of the membrane. 5. The membranous fraction is characterized by a high specific activity of alkaline phosphatase, trehalase, glucoamylase, maltase and gamma-glutamyltranspeptidase.
摘要

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