Cheng Jian-Hua, Tang Xiang-Yu, Liu Chen
Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Huan Jing Ke Xue. 2019 Jul 8;40(7):3257-3262. doi: 10.13227/j.hjkx.201811086.
Given the potential risk posed by antibiotic resistance genes (ARGs) to the eco-environment in the hilly purple soil region, which has been intensively utilized by human, surface soil samples were collected from feedlots of pig, chicken, and cattle farms and were analyzed for the diversity and abundance of ARGs using high-throughput QPCR. In total, 79 ARGs and 5 mobile genetic elements (MGEs) were detected across all samples. Among these genes, multidrug resistance genes were the most abundant type of ARGs. More abundant and diverse ARGs were observed in feedlot soil samples from pig and chicken farms than those from cattle feedlot soils, and these samples showed different distribution patterns of ARGs. High abundance of MGEs and their significant correlation with ARGs (<0.05) implied an important role of MGEs in the dissemination of ARGs in livestock feedlot soils.
鉴于抗生素抗性基因(ARGs)对人类高强度利用的丘陵紫色土地区生态环境构成潜在风险,采集了猪、鸡和牛养殖场饲养场的表层土壤样本,并使用高通量定量聚合酶链反应(QPCR)分析了ARGs的多样性和丰度。在所有样本中总共检测到79种ARGs和5种移动遗传元件(MGEs)。在这些基因中,多重耐药基因是ARGs中最丰富的类型。与牛饲养场土壤相比,猪和鸡养殖场饲养场土壤样本中观察到更丰富、更多样化的ARGs,并且这些样本显示出不同的ARGs分布模式。MGEs的高丰度及其与ARGs的显著相关性(<0.05)表明MGEs在畜禽饲养场土壤中ARGs传播中起重要作用。