Zhang Ru-Feng, Song Yuan, Gao Hao-Ze, Cheng Shou-Tao, Sun Yan-Mei, Wang Xu-Ming
College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Huan Jing Ke Xue. 2020 Jan 8;41(1):385-393. doi: 10.13227/j.hjkx.201907086.
The distribution characteristics of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in five vegetable base soils from Beijing, China, were assessed. The composition of ARGs and MGEs in soil samples were analyzed by HT-qPCR. We detected 92-121 ARGs and 4-6 MGEs. The ARGs and MGEs in vegetable base soils from different districts were separated from each other. The dominant ARGs shared by vegetable bases were , , and of a multidrug, of MLSB, and -Lactamase , of vancomycin. The shared MGE among the five vegetable base soils was . A total of seven antibiotics were detected in the soil of several vegetable bases. The dominant antibiotics included enoxacin (ENR), norfloxacin (NOR), oxytetracycline (OTC), and sulfamethoxazole (SMX). The numbers and abundance of antibiotics in the soil of vegetable bases from the Shunyi district were the highest, followed by those from Tongzhou and Changping. Correlation analysis showed that there was a significant positive correlation between the abundance of ARGs and the abundance of antibiotics in the soil of vegetable bases (<0.05). These results provide basic theoretical data for controlling the transmission of ARGs.
评估了中国北京五个蔬菜基地土壤中抗生素抗性基因（ARGs）和可移动遗传元件（MGEs）的分布特征。通过高通量定量聚合酶链反应（HT-qPCR）分析土壤样品中ARGs和MGEs的组成。我们检测到92 - 121个ARGs和4 - 6个MGEs。不同区蔬菜基地土壤中的ARGs和MGEs相互分离。蔬菜基地共有的优势ARGs为多药耐药的 、 、 ，大环内酯-林可酰胺-链阳霉素B（MLSB）类的 ，以及万古霉素的β-内酰胺酶 。五个蔬菜基地土壤中共有的MGE是 。在几个蔬菜基地的土壤中总共检测到七种抗生素。优势抗生素包括依诺沙星（ENR）、诺氟沙星（NOR）、土霉素（OTC）和磺胺甲恶唑（SMX）。顺义区蔬菜基地土壤中抗生素的数量和丰度最高，其次是通州区和昌平区。相关性分析表明，蔬菜基地土壤中ARGs的丰度与抗生素的丰度之间存在显著正相关（<0.05）。这些结果为控制ARGs的传播提供了基础理论数据。
