Jia Yuan-Hang, Jin Zhen-Jiang, Yuan Wu, Cheng Yue-Yang, Qiu Jiang-Mei, Liang Jin-Tao, Pan Fu-Jing, Liu De-Shen
College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China.
Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China.
Huan Jing Ke Xue. 2019 Jul 8;40(7):3313-3323. doi: 10.13227/j.hjkx.201811048.
In order to explore the effect of land-use change on soil bacteria in wetland systems, the topsoil (0-20 cm) of a natural wetland (NW), paddy field (PF), and dry land (DL) were collected in the Huixian karst wetland. The -diversity, species composition, and abundance of soil bacterial communities were analyzed using high-throughput sequencing. The effect of environmental factors on bacterial community structure was also examined. The results showed that the soil bacteria in the Huixian karst wetland can be divided into 49 phyla and 145 classes. The Shannon index of bacteria in the PF was significantly higher, and the Simpson index of bacteria in the NW is significantly lower, than in the other two land-use types. The dominant phyla (operational taxonomic units, OTUs>1%) in the NW were Proteobacteria (52.15%), Actinobacteria (15.16%), and Acidobacteria (8.80%); the dominant phyla in the PF were Proteobacteria (45.79%), Acidobacteria (17.20%), and Chloroflexi (11.75%); the dominant phyla in the DL were Proteus (51.42%), Acidobacteria (15.51%), and Chloroflexi (7.43%). The dominant classes (OTUs>1%) in the NW were -Proteobacteria (17.98%), -Proteobacteria (13.72%), and Actinobacteria (13.13%); the dominant classes in the PF were Acidobacteria (14.35%), -Proteobacteria (13.37%), and -Proteobacteria (12.02%); the dominant classes in the DL were -Proteobacteria (19.44%), Formobacteria (13.30%), and Acidobacteria (13.03%). Among the dominant OTUs (>0.3%), the dominant genera of in the NW were (OTU2, 59), (OTU5, 24 and 50487), (OTU1), and (OTU8); the dominant genera in the PF were (OTU4 and 115) and (OTU33); the dominant genera in the DL were (OTU85, 157 and 2916), (OTU19 and 52), and (OTU60). A heatmap showed that there were significant differences in soil bacterial community structure among the three land-use types. Redundancy analysis showed that pH, soil organic carbon (SOC), total nitrogen (TN), alkali-hydrolyzable nitrogen (AN), exchangeable Mg, exchangeable Ca, soluble organic carbon (DOC), and available phosphorus (AP) were the main factors that affected the bacterial community structure in the Huixian karst wetland. These results indicate that changes in land-use types have significantly shaped the structure of soil bacterial communities in this area.
为探究土地利用变化对湿地系统土壤细菌的影响，在辉县喀斯特湿地采集了天然湿地（NW）、稻田（PF）和旱地（DL）的表层土壤（0 - 20厘米）。采用高通量测序分析土壤细菌群落的多样性、物种组成和丰度。还研究了环境因素对细菌群落结构的影响。结果表明，辉县喀斯特湿地的土壤细菌可分为49个门和145个纲。与其他两种土地利用类型相比，PF中细菌的香农指数显著更高，NW中细菌的辛普森指数显著更低。NW中优势门（操作分类单元，OTUs>1%）为变形菌门（52.15%）、放线菌门（15.16%）和酸杆菌门（8.80%）；PF中优势门为变形菌门（45.79%）、酸杆菌门（17.20%）和绿弯菌门（11.75%）；DL中优势门为变形菌属（51.42%）、酸杆菌门（15.51%）和绿弯菌门（7.43%）。NW中优势纲（OTUs>1%）为γ-变形菌纲（17.98%）、β-变形菌纲（13.72%）和放线菌纲（13.13%）；PF中优势纲为酸杆菌纲（14.35%）、γ-变形菌纲（13.37%）和β-变形菌纲（12.02%）；DL中优势纲为γ-变形菌纲（19.44%）、芽单胞菌纲（13.30%）和酸杆菌纲（13.03%）。在优势OTUs（>0.3%）中，NW中的优势属为（OTU2，59）、（OTU5，24和50487）、（OTU1）和（OTU8）；PF中的优势属为（OTU4和115）和（OTU33）；DL中的优势属为（OTU85，157和2916）、（OTU19和52）和（OTU60）。热图显示三种土地利用类型的土壤细菌群落结构存在显著差异。冗余分析表明，pH、土壤有机碳（SOC）、总氮（TN）、碱解氮（AN）、交换性镁、交换性钙、可溶性有机碳（DOC）和有效磷（AP）是影响辉县喀斯特湿地细菌群落结构的主要因素。这些结果表明，土地利用类型的变化显著塑造了该地区土壤细菌群落的结构。
