BioPol, Chemistry Department, Federal University of Paraná, Caixa Postal 19081, Curitiba, PR 81531-980, Brazil.
Biochemistry and Molecular Biology Department, Federal University of Paraná, Caixa Postal 19046, Curitiba, PR 81531-980, Brazil.
Int J Biol Macromol. 2020 Feb 1;144:527-535. doi: 10.1016/j.ijbiomac.2019.12.114. Epub 2019 Dec 16.
Hyaluronic acid (HA) and galactomannans (such as guar gum - GG and locust bean gum - LBG) are biomacromolecules with various applications due to their physicochemical characteristics in solution, especially their ability to form films or hydrogels in some conditions. Some of these applications are possible because they are nontoxic and biocompatible. Mixtures of HA and GG or LBG, at pH 7.4, were prepared at 25 g·L of HA:LBG or HA:GG with percentages of 50:50, 60:40, 70:30 and 80:20 (w/w) and evaluated by rheology and NMR experiments regarding the interactions. The best synergism observed was between HA:LBG, most pronounced at 50% (w/w), where the dynamic modulus values G' and G″ indicated the formation of physical gels with viscoelastic behavior close to that of HA. 2D NOESY spectra revealed hydrogen interconnectivity of H-1 glucuronic acid units of HA with H-5 mannose units of the main galactomannan chains. This synergism, besides producing a hydrogel with interesting properties, also can decrease the cost by partially replacing HA in many applications, such as in nutraceutical foods, support for tissue engineering and cosmetics.
透明质酸（HA）和半乳甘露聚糖（如瓜尔胶-GG 和刺槐豆胶-LBG）是具有各种应用的生物大分子，这是由于它们在溶液中的物理化学特性，特别是在某些条件下形成薄膜或水凝胶的能力。这些应用中的一些是可能的，因为它们是无毒和生物相容的。在 pH 7.4 下，将 HA 和 GG 或 LBG 的混合物在 25 g·L 的 HA:LBG 或 HA:GG 下制备，其中 50:50、60:40、70:30 和 80:20（w/w），并通过流变学和 NMR 实验评估相互作用。观察到的最佳协同作用是在 HA:LBG 之间，在 50%（w/w）时最为明显，其中动态模量值 G'和 G″表明形成具有接近 HA 的粘弹性行为的物理凝胶。2D NOESY 光谱揭示了 HA 的 H-1 葡萄糖醛酸单元与主要半乳甘露聚糖链的 H-5 甘露糖单元之间的氢键连接。这种协同作用不仅可以产生具有有趣性质的水凝胶，还可以通过在许多应用中部分替代 HA 来降低成本，例如在营养食品、组织工程和化妆品中。
