Department of Zoology, University of Delhi, Delhi, India.
Department of Entomology, University of Agricultural Sciences, Raichur, India.
Int J Radiat Biol. 2020 Apr;96(4):532-544. doi: 10.1080/09553002.2020.1707323. Epub 2020 Jan 9.
The impact of gamma radiation on the reproductive biology of the spotted pod borer, (Fabr.) (Lepidoptera: Crambidae) was ascertained to explore the potential of the radio-genetic 'Inherited (F) Sterility' technique (modified Sterile Insect technique) to control this serious pigeonpea pest in India. Radio-biological investigations involved dose-response studies, at a range of 100-250 Gy, with respect to radiation induced adverse effects on reproductive behavior in substerilized parent (P) moths, inheritance of sterility and associated metamorphic disruption in F progeny. The reproductive competence of P male moths and their F progeny was evaluated by ascertaining the survival, mating success, sperm transfer and amphimixis of gametes from irradiated male parents and their F progeny. Daily ovipositional and egg fertility profile were evaluated as crucial parameters for simulation modeling for this radio-genetic technique. Radiation-induced check in insemination rate and sterility in P and F generation had a positive correlation with gamma dose. The metamorphic adverse effects in F progeny of substerilized P males were influenced by P irradiation. A higher dose of 200 Gy that resulted in 21.1% fertility in parent cross (P♂x N♀) could form only 14.4% F adults that were all malformed. A gamma dose of 150 Gy (administered to P males) could be considered as a suitable dose for F sterility, that induced 57.4% control of reproduction in parent generation, followed by 72-85% control of reproduction in F progeny involved in three crosses -F♂xN♀, N♂xF♀, F♂xF♀. Daily fertility profile of the eggs laid indicated a marked reduction on 4th day onwards during the ovipositional span in P and F generation. High degree of mating competitiveness value (CV) was noticed for 150 Gy irradiated male P moths (0.78-0.93), and their F male progeny (0.70-0.89), released in different sex ratios in the field cages having untreated moths. Reduced relative damage to the pigeon pea plants by the released F progeny in the field cages was observed in comparison to control. The present study indicated the potential use of a dose of 150 Gy to apply the 'F sterility' technique for parabiological suppression of the pigeon pea pod borer. The present findings and reproductive performance of the irradiated moths in F generation along with other compatible biorational pest control tactics (that are in progress), might help in formulating an effective integrated pest management module, including the F sterility technique as an integral component.
为了探索放射性遗传“可遗传（F）不育”技术（改良不育昆虫技术）在印度控制这种严重的斑豆蛀虫的潜力，确定了伽马辐射对斑豆蛀虫（Fabr.）（鳞翅目：螟蛾科）生殖生物学的影响。放射性生物学研究涉及剂量反应研究，范围为 100-250Gy，以研究辐射对亚不育亲代（P）蛾生殖行为、F 后代不育和相关变态破坏的不利影响。通过确定来自辐照雄性亲本及其 F 后代的精子存活率、交配成功率、精子转移和配子的雌雄同体，评估 P 雄性蛾及其 F 后代的生殖能力。每天产卵和卵育性概况被评估为这种放射性遗传技术模拟建模的关键参数。P 和 F 代的辐射诱导检查受精率和不育率与伽马剂量呈正相关。亚不育 P 雄虫的 F 后代的变态不良影响受 P 照射的影响。导致亲本杂交（P♂xN♀）中 21.1%可育的 200Gy 更高剂量仅能形成 14.4%的 F 成虫，且全部为畸形。150Gy（施用于 P 雄虫）的伽马剂量可被视为 F 不育的合适剂量，可诱导亲本代中 57.4%的繁殖控制，随后在涉及三个杂交的 F 后代中实现 72-85%的繁殖控制-F♂xN♀、N♂xF♀、F♂xF♀。P 和 F 代产卵期间的每日育性概况表明，在产卵期间的第 4 天开始显著减少。在田间笼中释放的经 150Gy 辐照的 P 雄蛾（0.78-0.93）及其 F 雄后代（0.70-0.89）具有较高的交配竞争力值（CV）。与对照相比，在田间笼中释放的 F 后代对羽扇豆植物的相对损害较小。本研究表明，可使用 150Gy 的剂量应用“F 不育”技术对斑豆蛀虫进行副生物抑制。目前的研究结果以及 F 代辐照雄蛾的生殖表现以及其他兼容的生物理性害虫控制策略（正在进行中），可能有助于制定有效的综合害虫管理模块，包括 F 不育技术作为一个组成部分。
