Department of Preventive Medicine, Public Health and Microbiology of the Autonomous University of Madrid, Madrid, Spain.
Becton-Dickinson &Co, Franklin Lakes, NJ, USA.
J Appl Microbiol. 2020 May;128(5):1339-1346. doi: 10.1111/jam.14559. Epub 2019 Dec 30.
ChloraPrep™ (CHP) is a clear solution of 2% (w/v) chlorhexidine (CHG) in 70% (v/v) isopropyl alcohol (IPA) administered with a specially designed sterile single-use applicator in which a tinting agent can be added to the CHP solution upon activation of applicator immediately prior to patient skin preparation (CHP+T). This study investigated whether the immediate and residual efficacy of CHP vs CHP+T and a stock solution of 2% CHG in 70% IPA varied, and whether CHP was compromised by the addition of the dye.
We compared the immediate and residual activity (in 1 min) of 70% IPA with that of 2% CHG in 70% IPA stock solution prepared in the laboratory against CHP+T and CHP, against 22 micro-organisms (5 ATCC and 18 clinical isolates) on germ-carriers. CHP and CHP+T demonstrated superior immediate and residual efficacy compared to the 70% IPA plus 2% CHG in 70% IPA stock solutions. Each antiseptic tested showed greater efficacy against the Gram-positive bacteria than against the Gram-negative bacteria. However, their antimicrobial effect on yeasts was even lower.
CHP and CHP+T have superior immediate and residual efficacy compared to stock 70% IPA and 2% CHG in 70% IPA solutions, and CHP+T is not affected by the tinting agent.
ChloraPrep is a product which can be stained just before use. We have demonstrated that the immediate and residual efficacy of the antimicrobial solution is not compromised by the dye. The efficacy of CHP is greater against bacteria than against yeasts obtained from ICU patients. Interestingly, CHP is more effective against bacteria than a formula made in the laboratory with the same basic components (2% chlorhexidine and 70% IPA). The intermittent heat sterilization process of the commercial preparation might hypothetically have improved the residual activity of the CHP solutions.
ChloraPrep™（CHP）是一种 2%（w/v）洗必泰（CHG）在 70%（v/v）异丙醇（IPA）中的透明溶液，用专门设计的无菌一次性给药器给药，在给药器激活时可以向 CHP 溶液中添加着色剂，立即在患者皮肤准备之前（CHP+T）。本研究旨在探讨 CHP 与 CHP+T 和 2% CHG 在 70% IPA 储备溶液之间的即时和残留效果是否不同，以及添加染料是否会影响 CHP 的效果。
我们比较了 70%IPA 与实验室制备的 2% CHG 在 70%IPA 储备溶液之间的即时和残留活性（1 分钟），并与 CHP+T 和 CHP 一起用于携带细菌的 22 种微生物（5 种 ATCC 和 18 种临床分离株）。CHP 和 CHP+T 与 70%IPA 加 2% CHG 在 70%IPA 储备溶液相比，具有更好的即时和残留效果。每种消毒剂对革兰氏阳性菌的效果均优于革兰氏阴性菌。然而，它们对酵母菌的抗菌作用甚至更低。
CHP 和 CHP+T 与 70%IPA 储备溶液和 2% CHG 在 70%IPA 溶液相比，具有更好的即时和残留效果，而且 CHP+T 不受着色剂的影响。
ChloraPrep 是一种可以在使用前染色的产品。我们已经证明，抗菌溶液的即时和残留效果不受染料的影响。CHP 对 ICU 患者分离的细菌的效果大于对酵母菌的效果。有趣的是，CHP 对细菌的效果比实验室用相同基本成分（2%洗必泰，70%IPA）制成的配方更强。商业制剂的间歇性热灭菌过程可能会使 CHP 溶液的残留活性得到改善。
