State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Int J Mol Sci. 2019 Dec 19;21(1):35. doi: 10.3390/ijms21010035.
Maize chlorotic mottle virus (MCMV) has been occurring frequently worldwide and causes severe yield losses in maize (). To better investigate the destructive effects of MCMV infection on maize plants, isobaric tagging for relative and absolute quantitation (iTRAQ)-based comparative proteomic analysis was performed on MCMV infected maize cv. B73. A total of 972 differentially abundant proteins (DAPs), including 661 proteins with increased abundance and 311 proteins with reduced abundance, were identified in response to MCMV infection. Functional annotations of DAPs and measurement of photosynthetic activity revealed that photosynthesis was decreased, while the abundance of ribosomal proteins, proteins related to stress responses, oxidation-reduction and redox homeostasis was altered significantly during MCMV infection. Two DAPs, disulfide isomerases like protein ZmPDIL-1 and peroxiredoxin family protein ZmPrx5, were further analyzed for their roles during MCMV infection through cucumber mosaic virus-based virus-induced gene silencing (CMV-VIGS). The accumulation of MCMV was suppressed in ZmPDIL-1-silenced or ZmPrx5-silenced B73 maize, suggesting ZmPDIL-1 and ZmPrx5 might enhance host susceptibility to MCMV infection.
玉米褪绿斑驳病毒（MCMV）在世界范围内频繁发生，导致玉米严重减产。为了更好地研究 MCMV 感染对玉米植株的破坏性影响，对 MCMV 感染的玉米 cv. B73 进行了基于等重标记相对和绝对定量（iTRAQ）的比较蛋白质组学分析。共鉴定出 972 个差异丰度蛋白（DAP），包括 661 个丰度增加的蛋白和 311 个丰度降低的蛋白，这些蛋白对 MCMV 感染有反应。DAP 的功能注释和光合作用活性的测量表明，光合作用减少，而核糖体蛋白、与应激反应、氧化还原和氧化还原稳态相关的蛋白的丰度在 MCMV 感染过程中发生了显著改变。通过基于黄瓜花叶病毒的病毒诱导基因沉默（CMV-VIGS），进一步分析了两个 DAP，即二硫键异构酶样蛋白 ZmPDIL-1 和过氧化物酶家族蛋白 ZmPrx5，以研究它们在 MCMV 感染过程中的作用。在 ZmPDIL-1 沉默或 ZmPrx5 沉默的 B73 玉米中，MCMV 的积累受到抑制，这表明 ZmPDIL-1 和 ZmPrx5 可能增强了宿主对 MCMV 感染的敏感性。
Zotero 插件
