用温和乙醇辅助的碱性过氧化物预处理来最大化竹子的酶水解效率。
Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment.
机构信息
Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China; Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN 37996, USA.
Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
出版信息
Bioresour Technol. 2020 Mar;299:122568. doi: 10.1016/j.biortech.2019.122568. Epub 2019 Dec 7.
To overcome the delignification saturation point in traditional alkaline hydrogen peroxide pretreatment (AHP), a powerful modified AHP delignification methodology was established by introducing ethanol into the system. The pretreatment caused significant lignin removal of bamboo at elevated pretreatment temperature with the highest lignin removal reaching 80.0% at 100 °C, higher than that (74.9% lignin removal) in pretreatment without the ethanol assistance. In addition, a certain amount of carbohydrates was also solubilized during the process whose recovery was 83.3% (glucan) and 67.6% (hemicellulose), respectively. The pretreated solid exhibited excellent enzymatic digestibility, with hydrolysis yields of ~100% and 95.7% for glucan and xylan, respectively. Our studies further indicate that this delignification methodology is versatile for hardwood and herbaceous plants, but does not perform well on softwood.
为了克服传统碱性过氧化氢预处理（AHP）中的脱木质素饱和点，通过向系统中引入乙醇，建立了一种强大的改良 AHP 脱木质素方法。该预处理方法在升高的预处理温度下可显著去除竹子中的木质素，在 100°C 时木质素去除率最高可达 80.0%，高于无乙醇辅助预处理时的木质素去除率（74.9%）。此外，在这个过程中还会溶解一定量的碳水化合物，其回收率分别为 83.3%（葡聚糖）和 67.6%（半纤维素）。预处理后的固体具有极好的酶解可及性，其葡萄糖和木聚糖的水解产率分别约为 100%和 95.7%。我们的研究进一步表明，这种脱木质素方法对硬木和草本植物具有通用性，但对软木的效果不佳。
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