Department of Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.
Tianjin Key Laboratory of Pulp & Paper, Tianjin University of Science and Technology, Tianjin 300457, China.
Bioresour Technol. 2018 Jan;247:242-249. doi: 10.1016/j.biortech.2017.09.080. Epub 2017 Sep 14.
A sequential two-stage pretreatment process comprising alkaline pre-extraction and alkaline hydrogen peroxide pretreatment (AHP) was investigated to convert bamboo carbohydrates into bioethanol. The results showed that mild alkaline pre-extraction using 8% (w/w) sodium hydroxide (NaOH) at 100°C for 180min followed by AHP pretreatment with 4% (w/w) hydrogen peroxide (HO) was sufficient to generate a substrate that could be efficiently digested with low enzyme loadings. Moreover, alkali pre-extraction enabled the use of lower HO charges in AHP treatment. Two-stage pretreatment followed by enzymatic hydrolysis with only 9FPU/g cellulose led to the recovery of 87% of the original sugars in the raw feedstock. The use of the pentose-hexose fermenting Saccharomyces cerevisiae SR8u strain enabled the utilization of 95.7% sugars in the hydrolysate to reach 4.6%w/v ethanol titer. The overall process also enabled the recovery of 62.9% lignin and 93.8% silica at high levels of purity.
采用两段式预处理工艺，包括碱性预提取和碱性过氧化物预处理（AHP），将竹材碳水化合物转化为生物乙醇。结果表明，温和的碱性预提取采用 8%（w/w）氢氧化钠（NaOH）在 100°C 下处理 180min，然后用 4%（w/w）过氧化氢（HO）进行 AHP 预处理，足以生成可在低酶用量下高效消化的底物。此外，碱预处理使 AHP 处理中可以使用较低的 HO 用量。两段式预处理后，仅用 9FPU/g 纤维素进行酶水解，即可从原料中回收 87%的原始糖。使用戊糖-己糖发酵酿酒酵母 SR8u 菌株可使水解液中 95.7%的糖达到 4.6%w/v 的乙醇浓度。该工艺还可在高纯度水平下回收 62.9%的木质素和 93.8%的二氧化硅。
