[超滤管净化-超高效液相色谱-串联质谱法测定婴儿配方奶粉中的左旋肉碱、胆碱、牛磺酸]

[Determination of L-carnitine, choline, taurine in infant formula milk powder by ultrafiltration tube cleaning and ultra-performance liquid chromatography-tandem mass spectrometry].

作者信息

Li Liping, Zhao Rong, Fan Sai, Zhang Nan, Chen Dong, Wu Guohua

机构信息

Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing 100013, China.

出版信息

Wei Sheng Yan Jiu. 2019 Nov;48(6):988-992.

PMID:31875827

Abstract

OBJECTIVE

To establish a method for simultaneous determination of L-carnitine, choline, taurin in infant by ultrafiltration tube cleaning and ultraperformance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).

METHODS

The sample was dissolved in water and acid hydrolyzed, then adjusted pH with a sodium hydroxide solution. After ultrafiltration tube cleaning and centrifugation, the sample solution was separated on an amide column ACQUITY UPLC BEH Amide(2. 1 mm × 100 mm, 1. 7 μm) and detected by MS/MS under multiple reaction monitoring(MRM) mode. The quantification was performed by the internal standard calibration method.

RESULTS

The linear ranges were 1. 00-500 μg/L for L-carnitine and choline, 10-1000 μg/L for taurine with correlation coefficients of 0. 999. The mean recoveries were 89. 7%-107. 4% with relative standard deviations(RSD, n = 6) were2. 6%-8. 1%. The detection limits of the method were 0. 812 mg/kg for choline, 0. 623 mg/kg for L-carnitine and 9. 34 mg/kg for taurine, respectively.

CONCLUSION

This method is simple, accurate, reproducible and sensitivity.

摘要

目的

建立一种采用超滤管净化结合超高效液相色谱-串联质谱法（UPLC-MS/MS）同时测定婴幼儿体内左旋肉碱、胆碱、牛磺酸的方法。

方法

样品用水溶解并进行酸水解，然后用氢氧化钠溶液调节pH值。经超滤管净化和离心后，样品溶液在酰胺柱ACQUITY UPLC BEH Amide（2.1 mm×100 mm，1.7 μm）上进行分离，并在多反应监测（MRM）模式下通过MS/MS检测。采用内标校准法进行定量分析。

结果

左旋肉碱和胆碱的线性范围为1.00 - 500 μg/L，牛磺酸的线性范围为10 - 1000 μg/L，相关系数均为0.999。平均回收率为89.7% - 107.4%，相对标准偏差（RSD，n = 6）为2.6% - 8.1%。该方法的检测限分别为胆碱0.812 mg/kg、左旋肉碱0.623 mg/kg、牛磺酸9.34 mg/kg。