Department of Pharmacy , University of Naples "Federico II" , Via D. Montesano 49 , 80131 Naples , Italy.
Department of Chemical Science and Technologies , University of Rome "Tor Vergata" , Via della Ricerca Scientifica 1 , 00133 Rome , Italy.
Anal Chem. 2020 Jan 21;92(2):1674-1679. doi: 10.1021/acs.analchem.9b02560. Epub 2020 Jan 7.
The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused toward the development of emerging technologies, such as liquid biopsy. DNA-based biosensors have been largely applied in this direction, and currently, there are two main approaches based on target/probe hybridization reported in the literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost associated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method.
基于纸的电化学生物传感器作为强大的诊断工具在传感器领域引起了广泛关注。特别是，在复杂基质中检测核酸是一个热门话题，特别是在开发新兴技术（如液体活检）时。基于 DNA 的生物传感器在这方面得到了广泛应用，目前，文献中报道了两种主要的基于目标/探针杂交的方法，即信号 ON 和信号 OFF。在本技术说明中，这两种方法与纸基电极结合进行了评估，使用单链 DNA 作为模型靶标，针对乳腺癌中第 20 外显子的 H1047R(A3140G)错义突变。对两种系统的分析性能、检测方案和相关成本进行了详细比较，根据应用情况突出了各自的优缺点。本工作面向广泛的受众，特别是那些在即时护理领域的受众，旨在提供管理设计和开发阶段的专业知识，并优化基于纸张的检测方法用于核酸检测的平台。
