Wilson F R, Westrum L E, Broderson S H
Department of Neurological Surgery, University of Washington School of Medicine, Seattle 98195.
Synapse. 1988;2(4):341-52. doi: 10.1002/syn.890020402.
Acetylcholinesterase (AChE) activity was investigated in synaptic areas of the cat spinal trigeminal nucleus (pars interpolaris and pars caudalis) ipsilateral and contralateral to complete retrogasserian rhizotomy. Vibratome sections of tissue taken from animals of 1, 3, 6, 14, and 21 days survival were examined by electron microscopy following a histochemical reaction for AChE activity employing a method based on the Karnovsky-Roots technique for demonstrating reaction product. As degeneration progressed with survival time, enzymatic activity was initially reduced in synaptic clefts of injured afferent terminals and subsequently was enhanced throughout the extracellular space, including within synaptic clefts of possibly reinnervated sites. These changes in enzymatic activity with primary deafferentation are discussed in relation to the process of reinnervation, the development of neuronal hyperactivity, and possible noncholinergic functions of AChE.
在完全性半月神经节后根切断术同侧和对侧的猫脊髓三叉神经核（极间部和尾侧部）的突触区域研究了乙酰胆碱酯酶（AChE）活性。采用基于卡诺夫斯基-鲁茨技术的方法对AChE活性进行组织化学反应后，通过电子显微镜检查取自存活1、3、6、14和21天动物的组织振动切片，以显示反应产物。随着存活时间的延长，变性进展，酶活性最初在受损传入终末的突触间隙中降低，随后在整个细胞外空间增强，包括在可能重新支配部位的突触间隙内。结合重新支配过程、神经元活动亢进的发展以及AChE可能的非胆碱能功能，讨论了初级去传入后酶活性的这些变化。
