Utsalo S J, Mboto C I, Gemade E I, Nwangwa M A
Department of Medical Microbiology and Parasitology, University of Calabar, Nigeria.
Trans R Soc Trop Med Hyg. 1988;82(2):327-9. doi: 10.1016/0035-9203(88)90464-6.
One hundred and ten hard clams (Mercenaria spp.) harvested from the Calabar river estuary were examined for total platable heterotrophic bacteria and Vibrio spp. in their mantle fluids using estuarine salts agar and thiosulphate citrate bile salts sucrose agar plates respectively. The mean counts of heterotrophic bacteria and vibrios were 7.9 x 10(6) organisms/ml and 2.5 x 10(2) vibrios/ml respectively. The rate of vibrio infection was 58.2%. Of the clams positive for vibrios, 54 (84%) harboured dual infections (V. alginolyticus and V. parahaemolyticus) of the moderate grade (greater than or equal to 10(3) to 10(5) vibrios/ml). Single infections due to V. alginolyticus or V. parahaemolyticus, found in 16% of clams, were either low (less than 10(3) vibrios/ml), moderate (greater than or equal to 10(3) to 10(5) vibrios/ml), or high (greater than 10(5) vibrios/ml). Dual infections only were detected in clams with mantle fluid volumes greater than 30 ml.
从卡拉巴尔河河口采集了110只硬壳蛤（Mercenaria spp.），分别使用河口盐琼脂和硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂平板检测其外套膜液中的总可培养异养细菌和弧菌属。异养细菌和弧菌的平均计数分别为7.9×10⁶个/毫升和2.5×10²个弧菌/毫升。弧菌感染率为58.2%。在弧菌检测呈阳性的蛤中，54只（84%）存在中度（≥10³至10⁵个弧菌/毫升）的双重感染（溶藻弧菌和副溶血性弧菌）。在16%的蛤中发现的由溶藻弧菌或副溶血性弧菌引起的单一感染，其程度要么为低（<10³个弧菌/毫升）、中度（≥10³至10⁵个弧菌/毫升），要么为高（>10⁵个弧菌/毫升）。仅在具有大于30毫升外套膜液体积的蛤中检测到双重感染。
