棘阿米巴 SBDS，一个细胞骨架相关基因，在吞噬作用和包囊形成过程中高度表达。
The Acanthamoeba SBDS, a cytoskeleton-associated gene, is highly expressed during phagocytosis and encystation.
机构信息
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Clinical Laboratory of Tainan Municipal Hospital (Managed by Show Chwan Medical Care Corporation), Tainan, Taiwan.
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Parasitology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
出版信息
J Microbiol Immunol Infect. 2021 Jun;54(3):482-489. doi: 10.1016/j.jmii.2019.11.003. Epub 2019 Dec 14.
BACKGROUND/PURPOSE: Shwachman-Bodian-Diamond syndrome (SBDS) protein is widely present in eukaryotes from vertebrates to protozoa. However, there are several variants within species, and previous studies have shown evidence that they may have additional functions. There are two SBDS-related proteins in Acanthamoeba. One is an rRNA metabolism protein of the SBDS family (ACA1_142090), and the other is SBDS (ACA1_204560). Although there is a conserved SBDS domain in the Acanthamoeba SBDS (ACA1_204560; AcSBDS), its function has not been reported. The aims of this study were to characterize the expression of AcSBDS during phagocytosis and encystation.
METHODS
AcSBDS-specific primer was designed to amplify the genomic AcSBDS of Acanthamoeba ATCC-30010. The AcSBDS expression was examined using reverse transcription polymerase chain reaction (RT-PCR) and immunostaining after phagocytosis and encystation treatment.
RESULTS
In this study, we found that the mRNA expression level of AcSBDS increased rapidly and that alternative splice variants were detected during phagocytosis and encystation processes. The results of immunofluorescence staining showed that the AcSBDS proteins accumulated surrounding phagocytosed bacteria.
CONCLUSION
Our results suggest that AcSBDS may not only have ribosomal maturation features but also have cytoskeleton-associated functions related to phagocytosis and encystation.
背景/目的：Shwachman-Bodian-Diamond 综合征（SBDS）蛋白广泛存在于真核生物中，从脊椎动物到原生动物。然而，在同一物种内存在几种变体，先前的研究表明它们可能具有额外的功能。棘阿米巴中有两种与 SBDS 相关的蛋白质。一种是 SBDS 家族的 rRNA 代谢蛋白（ACA1_142090），另一种是 SBDS（ACA1_204560）。尽管棘阿米巴 SBDS（ACA1_204560；AcSBDS）中存在保守的 SBDS 结构域，但它的功能尚未报道。本研究的目的是研究 AcSBDS 在吞噬和包囊形成过程中的表达特征。
方法
设计 AcSBDS 特异性引物扩增棘阿米巴 ATCC-30010 的基因组 AcSBDS。在吞噬和包囊形成处理后，通过反转录聚合酶链反应（RT-PCR）和免疫染色检测 AcSBDS 的表达。
结果
在这项研究中，我们发现 AcSBDS 的 mRNA 表达水平在吞噬和包囊形成过程中迅速增加，并检测到选择性剪接变体。免疫荧光染色结果表明，AcSBDS 蛋白在吞噬的细菌周围聚集。
结论
我们的结果表明，AcSBDS 不仅具有核糖体成熟的特征，而且还具有与吞噬和包囊形成相关的细胞骨架相关功能。
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