Department of Environmental and Occupational Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Environ Sci Pollut Res Int. 2020 Mar;27(7):7430-7438. doi: 10.1007/s11356-019-07351-9. Epub 2019 Dec 28.
The widespread usage and ubiquitous distribution of triclocarban (3,4,4'-trichlorocarbanilide, TCC) have raised public concerns about its health effects. At present, there is little information about the genotoxicity of TCC. In this study, we used a battery of genotoxicity testing methods including salmonella reverse mutation test (Ames test), comet assay and micronucleus assay to detect the effects of TCC on gene mutation, DNA breakage, and chromosome damage. The results of Ames test showed that TCC at 0.1-1000 μg/plate did not significantly increase the number of revertant colonies in the four standard Salmonella typhimurium strains, i.e., TA97, TA98, TA100, and TA102, when compared to the vehicle control. The results from comet assay demonstrated that exposure to 5, 10, or 15 μM TCC for 24 h did not significantly increase the percentage of comet cells, tail length (TL), DNA in tail (T DNA%), or olive tail moment (OTM) in keratinocyte HaCaT and hepatic L02 cells. Moreover, TCC did not markedly enhance the frequency of micronucleated cells or micronuclei in HaCaT and L02 cells in the micronucleus assay. Taken together, the results indicated that TCC did not exhibit any genotoxic effects. Our study provides additional information for the safety profile of TCC.
三氯生（3,4,4'-三氯卡班，TCC）的广泛使用和无处不在的分布引起了公众对其健康影响的关注。目前，关于 TCC 的遗传毒性知之甚少。在这项研究中，我们使用了一系列遗传毒性测试方法，包括沙门氏菌回复突变测试（Ames 测试）、彗星试验和微核试验，以检测 TCC 对基因突变、DNA 断裂和染色体损伤的影响。Ames 测试的结果表明，与载体对照组相比，TCC 在 0.1-1000μg/平板时并未显著增加四个标准鼠伤寒沙门氏菌菌株（TA97、TA98、TA100 和 TA102）中的回复菌落数量。彗星试验的结果表明，暴露于 5、10 或 15μM TCC 24 小时不会显著增加角质形成细胞 HaCaT 和肝细胞 L02 细胞中的彗星细胞百分比、尾部长度（TL）、尾部 DNA（T DNA%）或橄榄尾矩（OTM）。此外，TCC 并未明显增加微核试验中 HaCaT 和 L02 细胞中微核细胞或微核的频率。总之，这些结果表明 TCC 没有表现出任何遗传毒性作用。我们的研究为 TCC 的安全性概况提供了更多信息。
