Increasing the dietary fiber contents in isomaltooligosaccharides by dextransucrase reaction with sucrose as a glucosyl donor.

Isomaltooligosaccharides (IMOs) have been widely used as alternative sweeteners owing to their stabilities, low calorigenic, and prebiotic properties. The aim of this research was to improve the functionality of conventionally produced IMOs by increasing dietary fiber (DF) content with newly synthesized α-(1,6)-linkages through the dextransucrase reaction. To optimize the reaction conditions, various combinations of IMO and sucrose concentrations were applied as acceptor and donor molecules, respectively. Soluble DF content in the enzymatically-modified IMOs increased significantly with the initial substrate mixture of 10 % sucrose and 20 % IMOs; both DF and IMO contents increased to 35 % and 54 %, respectively. It was clearly suggested a simple dextransucrase-involved bioprocess could be applied to increase the DF content to the IMOs produced via a conventional process without scarifying the original IMO contents. Thus, it will be expected that the DF-enhanced IMO products are potentially applicable as functional ingredients as sugar substitutes in the food industry.