Um Ha-Eun, Park Bo-Ram, Kim Young Min, Lee Byung-Hoo
Department of Food Science & Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.
Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 55365, Republic of Korea.
Food Chem. 2023 Aug 15;417:135892. doi: 10.1016/j.foodchem.2023.135892. Epub 2023 Mar 17.
Isomaltooligosaccharides (IMOs) are widely used as prebiotic ingredients that promote colon health; however, recent studies revealed that these are slowly hydrolyzed to glucose within the small intestine. Here, novel α-glucans with a higher number of α-1,6 linkages were synthesized from maltodextrins using the Thermoanaerobacter thermocopriae-derived transglucosidase (TtTG) to decrease susceptibility to hydrolysis and improve slow digestion properties. The synthesized long-sized IMOs (l-IMOs; 70.1% of α-1,6 linkages), comprising 10-12 glucosyl units, exhibited slow hydrolysis to glucose when compared to commercial IMOs under treatment with mammalian α-glucosidase level. In male mice, the ingestion of l-IMOs significantly decreased the post-prandial glycemic response compared to other samples (p < 0.05). Therefore, enzymatically synthesized l-IMOs can be applied as functional ingredients for the modulation of blood glucose homeostasis in obesity, Type 2 diabetes, and other chronic diseases.
异麦芽低聚糖（IMOs）作为促进结肠健康的益生元成分被广泛使用；然而，最近的研究表明，这些物质在小肠内会缓慢水解为葡萄糖。在此，使用源自嗜热栖热放线菌的转葡萄糖苷酶（TtTG）从麦芽糊精合成了具有更多α-1,6键的新型α-葡聚糖，以降低水解敏感性并改善缓慢消化特性。合成的长链IMO（l-IMOs；α-1,6键占70.1%）由10-12个葡萄糖基单元组成，与市售IMOs相比，在哺乳动物α-葡萄糖苷酶水平处理下，其向葡萄糖的水解速度较慢。在雄性小鼠中，与其他样品相比，摄入l-IMOs显著降低了餐后血糖反应（p < 0.05）。因此，酶促合成的l-IMOs可作为功能性成分用于调节肥胖、2型糖尿病和其他慢性疾病中的血糖稳态。
