QOPNA and LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
INRA, UMR408 Sécurité et Qualité des Produits d'Origine Végétale, INRA, Avignon University, F-84000 Avignon, France.
Carbohydr Polym. 2020 Feb 15;230:115644. doi: 10.1016/j.carbpol.2019.115644. Epub 2019 Nov 19.
Given the high prevalence of arabinan side chains in pectic polysaccharides, this work aims to unveil the impact of their structural diversity on pectic polysaccharides-polyphenol interactions. To assess the effect of arabinan branching degree, sugar beet arabinans (branched and debranched) were used and compared to the well-known structure of apple arabinan and other pectic polysaccharides. Furthermore, arabinans contribution to pectic polysaccharides/polyphenol interactions was assessed. The interactions were evaluated using chlorogenic acid, phloridzin and procyanidins (degree of polymerization of 9). Linear arabinans had 8-fold and 2-fold higher retention for chlorogenic acid and phloridzin, respectively, than branched arabinans. This trend was also observed for the interaction of arabinans with procyanidins. However, arabinans with covalently linked polyphenols showed lower interactions. The interactions involved between arabinans and polyphenols explained 1-28 % of the interactions of pectic polysaccharides, allowing us to conclude that the whole polysaccharide structure is more relevant for polyphenol interactions than each part.
鉴于阿拉伯聚糖侧链在果胶多糖中的高普遍性，本工作旨在揭示其结构多样性对果胶多糖-多酚相互作用的影响。为了评估阿拉伯聚糖支化程度的影响，使用了糖甜菜阿拉伯聚糖（支化和去支化），并将其与众所周知的苹果阿拉伯聚糖和其他果胶多糖的结构进行了比较。此外，还评估了阿拉伯聚糖对果胶多糖-多酚相互作用的贡献。使用绿原酸、根皮苷和原花青素（聚合度为 9）评估相互作用。线性阿拉伯聚糖对绿原酸和根皮苷的保留率分别比支化阿拉伯聚糖高 8 倍和 2 倍。这种趋势也观察到阿拉伯聚糖与原花青素的相互作用中。然而，与多酚共价结合的阿拉伯聚糖表现出较低的相互作用。阿拉伯聚糖与多酚之间的相互作用解释了果胶多糖相互作用的 1-28%，这使我们得出结论，与每个部分相比，整个多糖结构与多酚相互作用更为相关。
