大豆油和鱼油对奶牛皮下脂肪组织中脂质相关转录本的影响。

Effect of Soybean Oil and Fish Oil on Lipid-Related Transcripts in Subcutaneous Adipose Tissue of Dairy Cows.

作者信息

Vargas-Bello-Pérez Einar, Bionaz Massimo, Garrido-Sartore Macarena, Cancino-Padilla Nathaly, Morales María Sol, Romero Jaime, Leskinen Heidi, Garnsworthy Philip C, Loor Juan J

机构信息

Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Casilla 306, Santiago 6904411, Chile.

Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, DK-1870 Frederiksberg C, Denmark.

出版信息

Animals (Basel). 2019 Dec 26;10(1):54. doi: 10.3390/ani10010054.

DOI:10.3390/ani10010054

PMID:31888021

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7023039/

Abstract

The objective of this study was to determine the effect of long-term supplementation of unsaturated oil on lipid metabolism and transcription of genes involved in lipid metabolism in subcutaneous adipose tissue (SAT) of mid-lactating dairy cows. The objective was achieved by supplementing dairy cows with soybean oil (SO; high in linoleic acid) or fish oil (FO; high in EPA and DHA) for 63 days (nine weeks). Cows were fed a control diet with no added lipid, or diets containing SO or FO (n = 5 cows/group). At the onset of the experiment (day 0) and on days 21, 42, and 63 of supplementation, blood and SAT samples were collected from each animal. Oil supplementation increased cholesterol and NEFA in plasma, with a greater effect of SO compared to FO. Concentration of BUN was lower in SO compared to control and FO at the end of the trial. Transcription of few genes was affected by dietary lipids: had lowest expression in FO followed by SO and control. and had higher expression in FO. Transcription of was higher but expression of was lower in SO. Overall, results revealed that compared to control, SO and FO had lipogenic effect in SAT.

摘要

本研究的目的是确定长期补充不饱和油对泌乳中期奶牛皮下脂肪组织（SAT）脂质代谢及脂质代谢相关基因转录的影响。通过给奶牛补充大豆油（SO；富含亚油酸）或鱼油（FO；富含二十碳五烯酸和二十二碳六烯酸）63天（九周）来实现这一目标。给奶牛饲喂不添加脂质的对照日粮，或含有SO或FO的日粮（每组n = 5头奶牛）。在实验开始时（第0天）以及补充的第21、42和63天，从每头动物采集血液和SAT样本。补充油类可提高血浆中的胆固醇和非酯化脂肪酸（NEFA），与FO相比，SO的作用更大。试验结束时，与对照和FO相比，SO组的血尿素氮（BUN）浓度较低。少数基因的转录受日粮脂质影响：在FO中表达最低，其次是SO和对照。和在FO中表达较高。在SO中转录较高，但的表达较低。总体而言，结果表明，与对照相比，SO和FO在SAT中具有生脂作用。