de Jong B M, Ruijter J M, Romijn H J
Netherlands Institute for Brain Research, Amsterdam.
Int J Dev Neurosci. 1988;6(4):327-39. doi: 10.1016/0736-5748(88)90016-0.
Neocortex explants obtained from 6-day-old rat pups and cultured in a serum-free medium from 5 hr to 13 days in vitro (DIV) show preservation of cytoarchitectural characteristics. Major changes in the size of the explants and their layers occur during the first 2 DIV. A radial arrangement of neurons within layer 2-3-4, which becomes apparent between 2 and 10 DIV, suggests an advance in maturation in culture. In contrast to the situation in vivo, a distinct layer 4 cannot be consistently identified. During the first 2 DIV, a transposition of cells into the pial direction can be seen. Individual degenerated cells are spotted especially in layer 5. The presence of these cells amidst healthy neurons suggests that their death has a functional cause. Neurons at the ventricular border of layer 6 become relatively large in comparison with other neurons. Within the cultured tissue there is a marked increase in GFA reactivity compared to the situation in vivo. The described results clearly indicate that in these cultured explants, both similarities and differences are of interest for studies on the formation of neuronal circuitry within the cerebral cortex.
从6日龄大鼠幼崽获取的新皮质外植体，在无血清培养基中体外培养5小时至13天（体外培养天数，DIV），显示出细胞结构特征得以保留。外植体及其各层大小在最初2个DIV期间发生主要变化。2 - 3 - 4层内神经元的放射状排列在2至10个DIV之间变得明显，表明培养中成熟度有所进展。与体内情况相反，无法始终如一地识别出明显的第4层。在最初2个DIV期间，可观察到细胞向软膜方向移位。尤其在第5层发现个别退化细胞。这些细胞存在于健康神经元之中，表明它们的死亡有功能方面的原因。与其他神经元相比，第6层室管膜边界处的神经元变得相对较大。与体内情况相比，培养组织内胶质纤维酸性蛋白（GFA）反应性显著增加。所述结果清楚表明，在这些培养的外植体中，相似性和差异对于研究大脑皮质内神经回路的形成均具有重要意义。
