Gaucher Floriane, Bonnassie Sylvie, Rabah Houem, Leverrier Pauline, Pottier Sandrine, Jardin Julien, Briard-Bion Valérie, Marchand Pierre, Jeantet Romain, Blanc Philippe, Jan Gwénaël
UMR STLO, Agrocampus Ouest, INRA, F-35042, Rennes, France.
Bioprox, 6 rue Barbès, 92532, Levallois-Perret, France.
Data Brief. 2019 Dec 4;28:104932. doi: 10.1016/j.dib.2019.104932. eCollection 2020 Feb.
The article presents a proteomic data set generated by a comparative analysis of the proteomes of comparing the CIRM-BIA 129 and CIRM-BIA 1025 strains. The two strains were cultivated until the beginning of the stationary phase in a chemical defined medium (MMO), and in this medium in the presence of NaCl, with or without glycine betaine. Whole-cell proteins were extracted, trypsinolyzed and analyzed by nano LC-MS/MS, prior to identification and classification by function using the X!Tandem pipeline software and the proteomic data from NCBI.nlm.nigh.gov. Quantification of proteins was then carried out in order to detect change in their expression depending on the culture medium. This article is related to the research article entitled "Benefits and drawbacks of osmotic adjustment in ". The comparative proteomic analysis of the two strains reveal strain-dependent and medium-dependent stress proteomes in the probiotic .
本文展示了通过对CIRM - BIA 129和CIRM - BIA 1025菌株蛋白质组进行比较分析而生成的蛋白质组数据集。将这两种菌株在化学限定培养基（MMO）中培养至稳定期开始，并且在该培养基中加入NaCl，添加或不添加甘氨酸甜菜碱。提取全细胞蛋白质，进行胰蛋白酶消化并通过纳升液相色谱 - 串联质谱（nano LC - MS/MS）分析，然后使用X!Tandem管道软件和来自NCBI.nlm.nigh.gov的蛋白质组数据进行功能鉴定和分类。接着对蛋白质进行定量，以检测其表达随培养基的变化。本文与题为“渗透调节的利弊”的研究文章相关。对这两种菌株的比较蛋白质组分析揭示了益生菌中菌株依赖性和培养基依赖性应激蛋白质组。
