雌二醇 17β/miRNA-26a/cyp19a1a 在雌雄同体鱼类卵形鲳鲹中的调控反馈环。
An estradiol-17β/miRNA-26a/cyp19a1a regulatory feedback loop in the protogynous hermaphroditic fish, Epinephelus coioides.
机构信息
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266373, China; Southern Marine Science and Engineering Guangdong Laboratory (ZhanJiang), Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, PR China; Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China.
出版信息
Mol Cell Endocrinol. 2020 Mar 15;504:110689. doi: 10.1016/j.mce.2019.110689. Epub 2019 Dec 28.
Cyp19a1a is a key gene responsible for the production of estradiol-17β (E), the main functional estrogen and a major downstream regulator of reproduction in teleost fish. It is widely known that CYP19 gene expression, aromatase activity, and E production can influence gonadal differentiation and sex reversal in teleost fish, but the feedback mechanisms whereby E regulates cyp19a1a remain poorly understood, especially regarding the potential roles of endogenous small RNA molecules (miRNAs). Here, we identified miR-26a-5p as a regulatory factor of its predicted target gene (cyp19a1a). In vitro and in vivo studies showed that miR-26a-5p can decrease cyp19a1a expression. Furthermore, high doses of E act as a repressor of miR-26a-5p. This study proposes a regulatory feedback loop whereby E regulates cyp19a1a through miR-26a-5p, and suggests that this positive feedback is an important aspect of the control of E production.
Cyp19a1a 是一个关键基因，负责产生雌二醇-17β（E），E 是鱼类主要的功能性雌激素，也是生殖的主要下游调节剂。众所周知，CYP19 基因表达、芳香酶活性和 E 产生会影响鱼类的性腺分化和性反转，但 E 调节 cyp19a1a 的反馈机制仍知之甚少，特别是关于内源性小 RNA 分子（miRNA）的潜在作用。在这里，我们鉴定出 miR-26a-5p 是其预测靶基因（cyp19a1a）的调节因子。体外和体内研究表明，miR-26a-5p 可以降低 cyp19a1a 的表达。此外，高剂量的 E 可以作为 miR-26a-5p 的抑制剂。本研究提出了一个调节反馈回路，其中 E 通过 miR-26a-5p 调节 cyp19a1a，并表明这种正反馈是 E 产生控制的一个重要方面。
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