Department of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet, SE 17176 Solna, Sweden; Functional Materials Group, Department of Applied Physics, School of Engineering Sciences, KTH Royal Institute of Technology, SE-164 40 Stockholm, Sweden.
Department of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, Oslo 0316, Norway.
J Chromatogr A. 2020 Apr 12;1616:460790. doi: 10.1016/j.chroma.2019.460790. Epub 2019 Dec 14.
Biological samples are usually complex matrices due to the presence of proteins, salts and a variety of organic compounds with chemical properties similar to those of the target analytes. Therefore, sample preparation is often mandatory in order to isolate the analytes from troublesome matrices before instrumental analysis. Because the number of samples in drug development, doping analysis, forensic science, toxicological analysis, and preclinical and clinical assays is steadily increasing, novel high throughput sample preparation approaches are calling for. The key factors in this development are the miniaturization and the automation of the sample preparation approaches so as to cope with most of the twelve principles of green chemistry. In this review, recent trends in sample preparation and novel strategies will be discussed in detail with particular focus on sorptive and liquid-phase microextraction in bioanalysis. The actual applicability of selective sorbents is also considered. Additionally, the role of 3D printing in microextraction for bioanalytical methods will be pinpointed.
生物样本通常是复杂的基质，因为存在蛋白质、盐和各种具有与目标分析物相似化学性质的有机化合物。因此，在进行仪器分析之前，通常需要进行样品制备以将分析物从麻烦的基质中分离出来。由于药物开发、兴奋剂分析、法医学、毒理学分析以及临床前和临床检测中的样品数量稳步增加，因此需要新型高通量样品制备方法。这一发展的关键因素是样品制备方法的小型化和自动化，以应对大多数绿色化学十二条原则。在这篇综述中，将详细讨论样品制备的最新趋势和新策略，特别关注生物分析中的吸附和液相微萃取。还考虑了选择性吸附剂的实际适用性。此外，还将指出 3D 打印在生物分析方法中的微萃取中的作用。
