Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Cellular and Molecular Research Center, Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Nefrologia (Engl Ed). 2020 May-Jun;40(3):311-319. doi: 10.1016/j.nefro.2019.10.003. Epub 2019 Dec 28.
Renal ischemia-reperfusion occurs in some clinical conditions such as kidney surgery that can leads to acute renal failure. The aim of this study was to investigate the effect of p-coumaric acid (CA) on ischemia reperfusion (I/R) injury.
Thirty rats were randomly divided into five groups; control, CA (100mg/kg), I/R, propylene glycol (10%)+I/R and CA+I/R, (n=6 each). CA and propylene glycol were administered orally for 2 weeks. Then, the rats were subjected to bilateral renal ischemia for 45min and followed by reperfusion for 24h. All rats were killed and kidney function tests, tissue malondialdehyde and activity of antioxidant enzymes were determined. Histopathological evaluations were also performed. In addition, renal expression of the tumor necrosis factor-α and interleukin-1β were determined using enzyme-linked immunosorbent assay and immunohistochemistry.
CA significantly improved the Cr and BUN levels in CA+I/R group compared to I/R group (p<0.005 and p<0.001, respectively). Reduction of tissue superoxide dismutase, glutathione peroxidase and catalase, were significantly improved by CA (p<0.01, p<0.01 and p<0.05). Treatment with CA also resulted in significant reduction in tissue MDA (p<0.05), TNF-α (p<0.001) and interleukin-1β expression (p<0.001) that were increased by renal I/R. Also, the rats treated with CA had nearly normal structure of the kidney.
The present findings suggest that, CA protects the kidneys against I/R injury via its antioxidant and anti-inflammatory effects.
肾缺血再灌注发生于肾脏手术等一些临床情况中，可导致急性肾衰竭。本研究旨在探讨对香豆酸（CA）对缺血再灌注（I/R）损伤的影响。
30 只大鼠随机分为 5 组；对照组、CA（100mg/kg）组、I/R 组、10%丙二醇+I/R 组和 CA+I/R 组（每组 6 只）。CA 和丙二醇分别口服给药 2 周。然后，大鼠双侧肾脏缺血 45min，再灌注 24h。处死所有大鼠，测定肾功能，测定组织丙二醛和抗氧化酶活性。还进行了组织病理学评价。此外，还通过酶联免疫吸附试验和免疫组化法测定肾肿瘤坏死因子-α和白细胞介素-1β的表达。
与 I/R 组相比，CA+I/R 组 CA 显著改善了 Cr 和 BUN 水平（p<0.005 和 p<0.001）。CA 显著改善了组织超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的减少（p<0.01、p<0.01 和 p<0.05）。CA 治疗还导致组织 MDA（p<0.05）、TNF-α（p<0.001）和白细胞介素-1β表达（p<0.001）的显著减少，这些表达在肾 I/R 后增加。此外，用 CA 治疗的大鼠的肾脏结构几乎正常。
本研究结果表明，CA 通过其抗氧化和抗炎作用保护肾脏免受 I/R 损伤。
