Rodríguez-Rodríguez Diana Raquel, Mendoza-Hernández Oscar Humberto, Cordero-Pérez Paula, Rivas-Galindo Verónica Mayela, Moreno-Peña Diana Patricia, Tijerina-Márquez Ramiro, Garza-Villarreal Alondra Michelle, Alarcón-Galván Gabriela, Muñoz-Espinosa Linda Elsa, Zapata-Chavira Homero Arturo, Hernández-Guedea Marco Antonio, Solis-Cruz Guadalupe Yazmín, Torres-González Liliana
Liver Unit, Department of Internal Medicine, University Hospital "Dr. José E. González", Universidad Autónoma de Nuevo León, Monterrey 64460, Nuevo León, Mexico.
Department of Analytical Chemistry, School of Medicine, Universidad Autónoma de Nuevo León, Monterrey 64460, Nuevo León, Mexico.
Int J Mol Sci. 2025 Feb 21;26(5):1838. doi: 10.3390/ijms26051838.
Plant extracts with antioxidant activities have shown nephroprotection against IR injury. (Jd) possesses antioxidant activity. Our aim was to evaluate the effects of a hydroalcoholic Jd extract against IR injury in Wistar rats. Rats were divided into groups (n = 6): sham (SH); no toxicity (JdTox; 300 mg/kg/day of extract for 7 days); IR (on day 7 [I: 45 min/R: 24 h]); and Jd+IR (same treatment as JdTox; same surgical procedure as IR). AST and LDH were significantly lower in the JdTox. IR exhibited significantly higher CrS, BUN, and MDA compared with SH; Jd+IR showed reductions in these markers. GSH and SOD levels were significantly lower in IR compared with SH; an increase in these markers was observed in the Jd+IR. Histologically, IR showed significant increases in medullary tubular necrosis, medullary protein casts, and medullary vascular congestion compared with SH and JdTox. In Jd+IR, a significant decrease was observed only in medullary tubular necrosis. Therefore, the evaluated hydroalcoholic Jd extract dose showed no nephrotoxicity and hepatotoxicity. Jd extract pretreatment attenuated IR-induced renal injury, as evidenced by the improved serum markers of renal damage and oxidative stress.
具有抗氧化活性的植物提取物已显示出对缺血再灌注损伤的肾保护作用。（Jd）具有抗氧化活性。我们的目的是评估Jd水醇提取物对Wistar大鼠缺血再灌注损伤的影响。大鼠分为几组（n = 6）：假手术组（SH）；无毒性组（JdTox；每天300 mg/kg提取物，共7天）；缺血再灌注组（第7天[缺血：45分钟/再灌注：24小时]）；以及Jd+缺血再灌注组（与JdTox治疗相同；与缺血再灌注组手术过程相同）。JdTox组的谷草转氨酶（AST）和乳酸脱氢酶（LDH）显著降低。与SH组相比，缺血再灌注组的肌酐清除率（CrS）、血尿素氮（BUN）和丙二醛（MDA）显著升高；Jd+缺血再灌注组这些指标有所降低。与SH组相比，缺血再灌注组的谷胱甘肽（GSH）和超氧化物歧化酶（SOD）水平显著降低；Jd+缺血再灌注组这些指标有所升高。组织学上，与SH组和JdTox组相比，缺血再灌注组的髓质肾小管坏死、髓质蛋白管型和髓质血管充血显著增加。在Jd+缺血再灌注组中，仅髓质肾小管坏死显著减少。因此，所评估的Jd水醇提取物剂量未显示出肾毒性和肝毒性。Jd提取物预处理减轻了缺血再灌注诱导的肾损伤，肾损伤和氧化应激的血清标志物改善证明了这一点。
