分析小鼠转移灶的代谢

Analyzing the Metabolism of Metastases in Mice.

作者信息

Altea-Manzano Patricia, Broekaert Dorien, Duarte João A G, Fernández-García Juan, Planque Mélanie, Fendt Sarah-Maria

机构信息

Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, VIB, Leuven, Belgium.

Laboratory of Cellular Metabolism and Metabolic Regulation, Department of Oncology, KU Leuven and Leuven Cancer Institute (LKI), Leuven, Belgium.

出版信息

Methods Mol Biol. 2020;2088:93-118. doi: 10.1007/978-1-0716-0159-4_6.

DOI:10.1007/978-1-0716-0159-4_6

PMID:31893372

Abstract

Metastasis formation is the leading cause of death in cancer patients. It has recently emerged that cancer cells adapt their metabolism to successfully transition through the metastatic cascade. Consequently, measuring and analyzing the in vivo metabolism of metastases has the potential to reveal novel treatment strategies to prevent metastasis formation. Here, we describe two different metastasis mouse models and how their metabolism can be analyzed with metabolomics and C tracer analysis.

摘要

转移灶的形成是癌症患者死亡的主要原因。最近有研究表明，癌细胞会调整其代谢，以成功地完成转移级联反应。因此，测量和分析转移灶的体内代谢情况有可能揭示预防转移灶形成的新治疗策略。在此，我们描述了两种不同的转移小鼠模型，以及如何利用代谢组学和碳示踪分析来分析它们的代谢情况。