Siddique Muhammad Irfan, Tufail Saima, Ker Ze Heng, Khan Tahir Mehmood, Rasool Fatima, Sohail Muhammad Farhan, Shahid Nabeel
Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan/Centre for Drug Delivery Research, Faculty of Pharmacy, University Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan.
Pak J Pharm Sci. 2019 Sep;32(5(Supplementary)):2299-2304.
Chitosan nanoparticles (CSNPs) have proven their excellent drug delivery potential through various routes of administration and therefore, the need for large scale production of CSNPs for the commercialization is paramount. Their particle size and surface charge, drug loading capacity, and morphology were characterized in this study. Finally, drug release studies of both continuous and scalable modes were undertaken to ascertain suitability of CSNPs as a carrier for HC. The particle size of the large and small scale of HC-CSNPs was 253.3±16.4 nm and 225.4 ±9.6 nm, respectively. Besides, the surface charge of the large and small scale of HC-CSNPs was +35.3±0.3 mV and +32.6±2.5 mV, respectively. The size and surface charge of both HC-CSNPs were not proven to be statistically different. Drug loading capacity of large and small scale production of HC-CSNPs was high with 89%, and 83% of HC was loaded into CSNPs, respectively. Moreover, the morphology of both large and small scale production of HC-CSNPs had a similar shape and particle size. The drug release profile of CSNPs prepared by both methods showed a significantly (p<0.05) higher percentage release as compared to the free form. It is expected that positively charged nano-sized HC-CSNPs with high drug loading capacity could enhance the efficiency of drug delivery system to carry and diffuse into the target cells. The results obtained also suggested that the modified method applied could be further developed for large scale production of HC-CSNPs.
壳聚糖纳米颗粒（CSNPs）已通过多种给药途径证明了其出色的药物递送潜力，因此，大规模生产CSNPs以实现商业化至关重要。本研究对其粒径、表面电荷、载药量和形态进行了表征。最后，进行了连续和可扩展模式的药物释放研究，以确定CSNPs作为HC载体的适用性。大规模和小规模的HC-CSNPs的粒径分别为253.3±16.4 nm和225.4±9.6 nm。此外，大规模和小规模的HC-CSNPs的表面电荷分别为+35.3±0.3 mV和+32.6±2.5 mV。两种HC-CSNPs的大小和表面电荷经统计学检验无显著差异。大规模和小规模生产的HC-CSNPs的载药量都很高，分别有89%和83%的HC负载到CSNPs中。此外，大规模和小规模生产的HC-CSNPs的形态具有相似的形状和粒径。两种方法制备的CSNPs的药物释放曲线显示，与游离形式相比，释放百分比显著更高（p<0.05）。预计具有高载药量的带正电荷的纳米级HC-CSNPs可以提高药物递送系统携带并扩散到靶细胞中的效率。所得结果还表明，所应用的改进方法可进一步开发用于大规模生产HC-CSNPs。
