School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore637371.
Chem Commun (Camb). 2020 Jan 23;56(7):1082-1084. doi: 10.1039/c9cc06748e.
G-quadruplexes (G4) are non-canonical nucleic acid structures with important implications in biology. Based on an α-helical fragment of the RHAU helicase that displays high specificity for parallel-stranded G-quadrplexes, herein we demonstrate its head-to-tail cyclization by a high-efficiency ligase. The cyclic peptide exhibits superior stability and binding affinity to a G-quadruplex, and can serve as an excellent investigational tool for chemical biology applications.
G-四链体（G4）是具有重要生物学意义的非经典核酸结构。基于 RHAU 解旋酶的α-螺旋片段，该片段对平行链 G-四链体具有高度特异性，本文展示了其通过高效连接酶的头尾环化。该环状肽对 G-四链体表现出优异的稳定性和结合亲和力，可作为化学生物学应用的优秀研究工具。
