Beijing Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences, Beijing, 100083, People's Republic of China.
Geological Survey of Jiangsu Province, Nanjing, 210049, China.
Mikrochim Acta. 2020 Jan 2;187(1):86. doi: 10.1007/s00604-019-4065-6.
A novel electrochemical sensor is described for the determination of ofloxacin (OFL) in environmental water samples. A laser-modified glassy carbon electrode (LGCE) was structured and characterized by scanning electron microscopy, atomic force microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. The increase in electrochemical activity is due to a moderate increase in the surface roughness and to the presence of functional groups on the LGCE. Under optimal conditions (viz. a pH value of 5.5, a laser power of 1.8 W and an action time of 40 s), the sensor is capable of detecting OFL by differential pulse voltammetry at a working potential of +0.91 V (versus Ag/AgCl). Response is linear from 0.25 to 200 μM for OFL concentration range, and the detection limit is 75 nM (at S/N = 3). Removal of oxygen from samples is not required. The sensor was successfully applied to the determination of OFL in spiked groundwater, tap water and wastewater samples, with apparent recoveries from 94.0 to 108.0% and a relative standard deviation of less than 4.8%." Graphical abstractSchematic representation of a method for determination of ofloxacin (OFL) by differential pulse voltammetry. It is making use of a laser modified glassy carbon electrode (LGCE), which increases the number of active functional groups and the surface area compared to a conventional GCE.
一种用于测定环境水样中氧氟沙星（OFL）的新型电化学传感器。构建并通过扫描电子显微镜、原子力显微镜、X 射线衍射、X 射线光电子能谱和电化学阻抗谱对激光修饰玻碳电极（LGCE）进行了结构和特性表征。电化学活性的增加是由于 LGCE 表面粗糙度的适度增加以及存在官能团所致。在最佳条件下（即 pH 值为 5.5、激光功率为 1.8 W 和作用时间为 40 s），传感器能够通过差分脉冲伏安法在+0.91 V（相对于 Ag/AgCl）的工作电位下检测 OFL。对于 OFL 浓度范围，从 0.25 到 200 μM 响应呈线性，检测限为 75 nM（S/N = 3）。不需要从样品中去除氧气。该传感器成功应用于加标地下水、自来水和废水样品中 OFL 的测定，回收率为 94.0%至 108.0%，相对标准偏差小于 4.8%。
