Ruan Peng, Dai Xiufang, Sun Jun, He Chunping, Huang Chao, Zhou Rui, Chemin Isabelle
Department of Gastroenterology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan, 430060, Hubei, People's Republic of China.
Department of Breast Surgery, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, People's Republic of China.
Virus Genes. 2020 Apr;56(2):168-173. doi: 10.1007/s11262-019-01725-4. Epub 2020 Jan 2.
Integration of HBV DNA into host chromosomes was found in most of the patients with chronic hepatitis B (CHB). In this study, using inverse nested PCR (invPCR), we found the integration site chrX: 111,009,033, which inserted into the p21-activated kinase 3 (PAK3) gene in HepG2.2.15 cells. The viral-human chimeric transcripts were also observed and, significant differences of the copy numbers of integration site chrX: 111,009,033 (P = 0.012) and intra-cell HBV DNA levels (P = 0.027) were found between the cells with and without HO treatment, respectively. This study may provide a novel insight into the elucidation of etiology of HBV integration.
在大多数慢性乙型肝炎（CHB）患者中发现了乙肝病毒（HBV）DNA整合到宿主染色体的情况。在本研究中，我们使用反向巢式PCR（invPCR）在HepG2.2.15细胞中发现了整合位点chrX: 111,009,033，该位点插入到p21激活激酶3（PAK3）基因中。还观察到了病毒-人类嵌合转录本，并且在有和没有HO处理的细胞之间，分别发现整合位点chrX: 111,009,033的拷贝数（P = 0.012）和细胞内HBV DNA水平（P = 0.027）存在显著差异。本研究可能为阐明HBV整合的病因提供新的见解。
