植物组织中纳摩尔量氯离子的分光光度测定法。

The spectrophotometric determination of nanomole quantities of chloride in plant tissue.

作者信息

Allen C R, Allen S

机构信息

Department of Chemistry, University of Dundee, United Kingdom.

出版信息

Anal Biochem. 1988 Aug 15;173(1):54-8. doi: 10.1016/0003-2697(88)90158-3.

DOI:10.1016/0003-2697(88)90158-3

PMID:3189802

Abstract

The determination of Cl- in plant tissues was investigated by FeSCN2+ spectrophotometry following SCN- displacement from Hg(SCN)2 by Cl-. The calibration curve was nonlinear, but consistent and reproducible. As little as 10 nmol Cl- could be measured. There was no obvious interference in color development by water-soluble components in plant tissues. Values obtained by this method were in close agreement with those obtained potentiometrically and by coulometric titration.

摘要

通过Cl⁻将Hg(SCN)₂中的SCN⁻置换出来后，利用FeSCN²⁺分光光度法研究了植物组织中Cl⁻的测定。校准曲线是非线性的，但具有一致性和可重复性。最低可检测到10 nmol的Cl⁻。植物组织中的水溶性成分对显色没有明显干扰。该方法获得的值与电位滴定法和库仑滴定法获得的值非常一致。