Schneider R P, Zürcher F, Egli T, Hamer G
Institute of Aquatic Sciences, Swiss Federal Institute of Technology, Zürich.
Anal Biochem. 1988 Sep;173(2):278-84. doi: 10.1016/0003-2697(88)90190-x.
A sensitive method for the determination of nitrilotriacetate in biological growth media and cell-free extracts by ion exclusion chromatography is described using HCl as an eluant. The eluant conductivity was chemically suppressed with a membrane suppressor and a conductivity detector was used for subsequent detection. The membrane was continuously regenerated with a tetrabutylammoniumhydroxide solution. The detection limit for nitrilotriacetate in cell-free extract was 11 mg/liter, while for nitrilotriacetate in growth media it was 1 mg/liter. Interference by compounds present in biological matrices with the determination is discussed.
描述了一种通过离子排斥色谱法测定生物生长培养基和无细胞提取物中亚氨基三乙酸的灵敏方法，该方法使用盐酸作为洗脱液。洗脱液的电导率通过膜抑制器进行化学抑制，并使用电导检测器进行后续检测。膜用氢氧化四丁铵溶液连续再生。无细胞提取物中亚氨基三乙酸的检测限为11毫克/升，而生长培养基中亚氨基三乙酸的检测限为1毫克/升。讨论了生物基质中存在的化合物对测定的干扰。
