Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al Azhar University, Assiut, 71526, Egypt.
Anal Bioanal Chem. 2020 Feb;412(6):1353-1363. doi: 10.1007/s00216-019-02362-4. Epub 2020 Jan 4.
The study of biologically important Cu and S ions has drawn great attention in the recent years since an abnormal level of these ions is an indication for health impairment. Therefore, a reliable strategy for effective fluorescence determination of Cu and S ions was developed. Simply, the method based on economical plant-dependent thermolysis procedure for efficient green synthesis of water dispersible luminescent polyamine-based carbon dots (PA@C-dots) utilizes Vitis vinifera juice as precursor with a high quantum yield (32.1%) and good photo-stability. The fluorescent PA@C-dots were characterized by different spectroscopical, physical, and structural techniques. Furthermore, the synthesized PA@C-dots can be used as an efficient dual functional fluorescent probe for the sensitive and selective estimation of Cu and S ions. The incorporation of Cu ions and their adsorption on the surface of PA@C-dot skeleton leads to the respectable fluorescence quenching of C-dots (turn-off mode). The Cu-PA@C-dot was found to be sensitive to S ions. The addition of S recovers the fluorescence (turn-on mode) of Cu-PA@C-dots, thanks to its capacity for withdrawing Cu from the shell of PA@C-dots. Fluorescence quenching in the range of 0.07-60 μM Cu was obtained with LOD and LOQ of 0.02 and 0.066 μM, respectively. Sulfide detection provides linearity in the range of 0.8 to 95 μM with LOD and LOQ of 0.24 and 0.79 μM, respectively. The optimal excitation and emission wavelengths for all experiments are 435 nm and 498 nm, respectively. Experiment results elucidate that the proposed method is suitable for Cuand S ion detection in environmental water samples. Graphical abstract Green synthesis of polyamine-functionalized nanoprobe by thermolysis method from plant source as bifunctional sensing platform for determination of Cu and S in environmental water samples.
近年来，由于这些离子的异常水平是健康受损的一个迹象，因此对生物上重要的 Cu 和 S 离子的研究引起了极大的关注。因此，开发了一种用于有效荧光测定 Cu 和 S 离子的可靠策略。简单地说，该方法基于经济的植物依赖性热解程序，用于高效绿色合成水分散的荧光多胺基碳点 (PA@C-dots)，利用葡萄汁作为前体，具有高量子产率 (32.1%) 和良好的光稳定性。荧光 PA@C-dots 采用不同的光谱、物理和结构技术进行表征。此外，合成的 PA@C-dots 可用作高效的双功能荧光探针，用于灵敏和选择性估计 Cu 和 S 离子。Cu 离子的掺入及其在 PA@C-dot 骨架表面的吸附导致 C-dots 的可观荧光猝灭（关断模式）。发现 Cu-PA@C-dot 对 S 离子敏感。由于其从 PA@C-dots 壳中提取 Cu 的能力，添加 S 会恢复 Cu-PA@C-dots 的荧光（开启模式）。在 0.07-60 μM Cu 的范围内获得荧光猝灭，LOD 和 LOQ 分别为 0.02 和 0.066 μM。硫化物检测在 0.8 至 95 μM 的范围内提供线性，LOD 和 LOQ 分别为 0.24 和 0.79 μM。所有实验的最佳激发和发射波长分别为 435nm 和 498nm。实验结果表明，该方法适用于环境水样中 Cu 和 S 离子的检测。
