State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715, China; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400715, China.
J Invertebr Pathol. 2020 Feb;170:107322. doi: 10.1016/j.jip.2019.107322. Epub 2019 Dec 31.
Microsporidia are a fascinating phylum of obligate intracellular pathogens with unique infection processes and complicated life cycles. Microsporidian life cycles can be divided roughly into intracellular and extracellular stages. Currently, research on their life cycles were mainly explored by morphology because there are few molecular markers available with which to distinguish the different life stages. In this study, we generated H20, a monoclonal antibody (MAb) to label mature spores of Nosema bombycis. Immunofluorescence assays showed that the target protein of H20, which is highly stable and was barely affected by alkali and sodium dodecyl sulfate (SDS) treatments, was located on the mature spore surface. Western blot analysis showed that spore wall protein 26 (SWP26) was the likely target of H20. This MAb can specifically identify mature spores in a complex biological sample based on immunological detection of the parasite.
微孢子虫是一类具有独特感染过程和复杂生命周期的专性细胞内病原体，属于一门非常有趣的学科。微孢子虫的生命周期大致可以分为细胞内和细胞外阶段。目前，由于缺乏可用的分子标记物来区分不同的生命周期阶段，因此主要通过形态学来研究它们的生命周期。在这项研究中，我们生成了 H20，这是一种针对家蚕微孢子虫成熟孢子的单克隆抗体（MAb）。免疫荧光分析表明，H20 的靶蛋白高度稳定，几乎不受碱和十二烷基硫酸钠（SDS）处理的影响，位于成熟孢子的表面。Western blot 分析表明，孢子壁蛋白 26（SWP26）可能是 H20 的靶标。这种 MAb 可以基于对寄生虫的免疫学检测，在复杂的生物样本中特异性识别成熟孢子。
