高效液相色谱法快速测定血清中两性霉素B的含量，不受胆红素干扰。
Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin.
作者信息
Hosotsubo H, Takezawa J, Taenaka N, Hosotsubo K, Yoshiya I
机构信息
Central Laboratory for Clinical Investigation, Osaka University Hospital, Japan.
出版信息
Antimicrob Agents Chemother. 1988 Jul;32(7):1103-5. doi: 10.1128/AAC.32.7.1103.
Abstract
A high-performance liquid chromatographic method for the determination of amphotericin B concentrations in human serum without bilirubin interference was developed and compared with a microbiological assay. The high-performance liquid chromatographic assay utilized a reversed-phase trimethyl silica column, simple sample preparation, and visible detection. Reproducibility studies yielded coefficient-of-variation ranges from 1.02 to 2.11% for within-day precision and 2.88 to 4.32% for between-day precision. The correlation coefficient with the microbiological assay was 0.984 for amphotericin B.
摘要
建立了一种用于测定人血清中两性霉素B浓度且无胆红素干扰的高效液相色谱法，并与微生物测定法进行了比较。高效液相色谱测定法采用反相三甲硅烷基硅胶柱、简单的样品制备方法和可见光检测。重现性研究得出日内精密度的变异系数范围为1.02%至2.11%，日间精密度的变异系数范围为2.88%至4.32%。两性霉素B与微生物测定法的相关系数为0.984。
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