Forestry and Pomology Research Institute, Shanghai Key Lab of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
Genes Genomics. 2020 Apr;42(4):383-392. doi: 10.1007/s13258-019-00912-9. Epub 2020 Jan 4.
Parthenocarpy can be induced by gibberellin (GA) treatment in plants. The fruits of the loquat exhibit many seeds. GA treatment can induce the development of seedless fruit and increase fruit quality during production. However, the molecular mechanism of fruit setting under GA treatment is still unclear.
Our aim was to explore GA-induced parthenocarpy in triploid loquat by proteome analysis to identify the differentially expressed proteins.
A proteome analysis was performed using TMT protein labeling and LC-MS/MS in triploid loquat.
A total of 7290 protein groups were identified in the two stages of fruit setting. The quantitative results showed that 923 differentially expressed proteins (DEPs) were isolated, which were enriched in five pathways: ribosome, citrate cycle (TCA cycle), pentose phosphate, carbon metabolism, and carbon fixation. Twenty-four DEPs were annotated as putative key regulatory proteins involved in fruit setting, which were related to the auxin response, gibberellin metabolism, ethylene synthesis, and cell division. In addition, thirty-five DEPs were involved in the formation of the cell wall, which might be downstream proteins involved in cell proliferation during fruit setting.
Our report reveals new insight into the protein dynamics of loquat fruit setting induced by GA treatment via the analysis of proteome profiles and provides a reference for other Rosaceae species.
赤霉素（GA）处理可诱导植物单性结实。枇杷果实多籽，GA 处理可诱导无核果实发育，提高果实品质。然而，GA 处理下果实结实的分子机制尚不清楚。
通过蛋白质组分析探讨 GA 诱导三倍体枇杷单性结实，鉴定差异表达蛋白。
采用 TMT 蛋白标记和 LC-MS/MS 对三倍体枇杷的两个结实阶段进行蛋白质组分析。
在两个结实阶段共鉴定到 7290 个蛋白质组。定量结果显示，分离到 923 个差异表达蛋白（DEPs），富集于核糖体、柠檬酸循环（TCA 循环）、戊糖磷酸、碳代谢和碳固定 5 条通路。24 个 DEP 被注释为可能参与果实结实的关键调控蛋白，与生长素反应、赤霉素代谢、乙烯合成和细胞分裂有关。此外，35 个 DEP 参与细胞壁的形成，可能是果实结实过程中参与细胞增殖的下游蛋白。
本研究通过蛋白质组图谱分析，揭示了 GA 处理诱导枇杷果实结实的蛋白质动态变化新见解，为其他蔷薇科物种提供了参考。
